Title :

Comparative evaluation of DNA extraction methods for amplification by qPCR of superficial vs intracellular DNA from Bacillus spores

Author(s) :

Brauge, Thomas [Auteur]
Faille, Christine [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Inglebert, G [Auteur]
Dubois, Thomas [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Morieux, P [Auteur]
Slomianny, Christian [Auteur]
Midelet-Bourdin, Graziella [Auteur]

Journal title :

International Journal of Food Microbiology

Volume number :

266

Pages :

289-294

Publication date :

2018-02-02

HAL domain(s) :

Sciences du Vivant [q-bio]/Ingénierie des aliments
Physique [physics]/Matière Condensée [cond-mat]/Science des matériaux [cond-mat.mtrl-sci]
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire
Physique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
Sciences de l'ingénieur [physics]/Génie des procédés

English abstract : [en]

This study was designed to assess the efficiency of eight extraction methods regarding their ability to release superficial (exogenous) and intracellular (endogenous) DNA from B. cereus spores for subsequent analysis by ...
Show more >This study was designed to assess the efficiency of eight extraction methods regarding their ability to release superficial (exogenous) and intracellular (endogenous) DNA from B. cereus spores for subsequent analysis by quantitative PCR(qPCR). B.cereussporesShow less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
ENSCL
CNRS
INRA
Inserm

Collections :

• Physiologie Cellulaire (PHYCELL) - U1003
• Unité Matériaux et Transformations (UMET) - UMR 8207

Research team(s) :

Processus aux Interfaces et Hygiène des Matériaux (PIHM)

Submission date :

2019-05-17T09:15:09Z
2021-05-17T13:36:19Z