Titre :

TRPV6 Calcium Channel Targeting by Antibodies Raised against Extracellular Epitopes Induces Prostate Cancer Cell Apoptosis.

Auteur(s) :

Haustrate, A. [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Shapovalov, Georgii [Auteur]
Physiologie Cellulaire (PHYCELL) - U1003
Spriet, Corentin [Auteur]
Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Cordier, C. [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Kondratskyi, A. [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Noyer, L. [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Foulquier, Francois [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Prevarskaya, Natalia [Auteur]
Physiologie Cellulaire (PHYCELL) - U1003
Lehen'Kyi, V'Yacheslav [Auteur]
Physiologie Cellulaire (PHYCELL) - U1003
Noyer, L. [Auteur]

Titre de la revue :

Cancers

Nom court de la revue :

Cancers

Numéro :

15

Pagination :

1825

Date de publication :

2023

ISSN :

2072-6694

Mot(s)-clé(s) en anglais :

therapeutic antibody
TRPV6 channel
tumor targeting
apoptosis

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

The TRPV6 calcium channel is known to be up-regulated in various tumors. The efforts to target the TRPV6 channel in vivo are still ongoing to propose an effective therapy against cancer. Here, we report the generation of ...
Lire la suite >The TRPV6 calcium channel is known to be up-regulated in various tumors. The efforts to target the TRPV6 channel in vivo are still ongoing to propose an effective therapy against cancer. Here, we report the generation of two antibodies raised against extracellular epitopes corresponding to the extracellular loop between S1 and S2 (rb79) and the pore region (rb82). These antibodies generated a complex biphasic response with the transient activation of the TRPV6 channel. Store-operated calcium entry was consequently potentiated in the prostate cancer cell line LNCaP upon the treatment. Both rb79 and rb82 antibodies significantly decreased cell survival rate in a dose-dependent manner as compared to the control antibodies of the same isotype. This decrease was due to the enhanced cell death via apoptosis revealed using a sub-G1 peak in a cell cycle assay, TUNEL assay, and a Hoechst staining, having no effects in the PC3Mtrpv6−/− cell line. Moreover, all TUNEL-positive cells had TRPV6 membrane staining as compared to the control antibody treatment where TRPV6-positive cells were all TUNEL negative. These data clearly demonstrate that TRPV6 channel targeting using rb79 and rb82 antibodies is fatal and may be successfully used in the anticancer therapies.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Physiologie Cellulaire (PHYCELL) - U1003
• Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41
• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Date de dépôt :

2023-12-21T06:40:20Z
2024-02-19T09:36:27Z