Titre :

Brain Peri-Hematomal Area, a Strategic Interface for Blood Clearance: A Human Neuropathological and Transcriptomic Study.

Auteur(s) :

Puy, Laurent [Auteur]
Lille Neurosciences & Cognition (LilNCog) - U 1172
Perbet, Romain [Auteur]
Lille Neurosciences & Cognition - U 1172 [LilNCog]
Figeac, Martin [Auteur]
Genomic @ Lille - PLBS [GO@L]
Duchêne, Belinda [Auteur]
Cancer Heterogeneity, Plasticity and Resistance to Therapies (CANTHER) - UMR 9020 - UMR 1277
Deramecourt, Vincent [Auteur]
Lille Neurosciences & Cognition (LilNCog) - U 1172
Cordonnier, Charlotte [Auteur]
Lille Neurosciences & Cognition (LilNCog) - U 1172
BEREZOWSKI, Vincent [Auteur]
Lille Neurosciences & Cognition (LilNCog) - U 1172

Titre de la revue :

Stroke

Numéro :

53

Pagination :

2026–2035

Date de publication :

2022-04-27

ISSN :

1524-4628

Mot(s)-clé(s) en anglais :

cerebral hemorrhage
edema
hemoglobin
inflammation
macrophage
neuropathology

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Background: Enhancing the blood clearance process is a promising therapeutic strategy for intracerebral hemorrhage (ICH). We aimed to investigate the kinetic of this process after ICH in human brain tissue through the ...
Lire la suite >Background: Enhancing the blood clearance process is a promising therapeutic strategy for intracerebral hemorrhage (ICH). We aimed to investigate the kinetic of this process after ICH in human brain tissue through the monocyte-macrophage scavenger receptor (CD163)/HO-1 (hemoxygenase-1) pathway. Methods: We led a cross-sectional post-mortem study including 22 consecutive ICH cases (2005–2019) from the Lille Neurobank. Cases were grouped according to the time of death: ≤72 hours, 4 to 7 days, 8 to 15 days, 16 to 90 days, and >90 days after ICH onset. Paraffin-embedded tissue was extracted from 4 strategic areas, including hematoma core and peri-hematomal area to perform histological investigations. Additionally, we extracted RNA from the peri-hematomal area of 6 cases to perform transcriptomic analysis. Results: We included 19 ICH cases (median age: 79 [71–89] years; median delay ICH-death: 13 [5–41] days). The peri-hematomal area concentrated most of reactive microglia, CD163/HO-1 and iron deposits as compared with other brain areas. We found a surge in the blood clearance process from day 8 to day 15 after ICH onset. Transcriptomic analysis showed that HO-1 was the most upregulated gene (2.81±0.39, adjusted P=1.11×10–10) and CD163 the sixth (1.49±0.29, adjusted P=1.68×10–5). We also identified several upregulated genes that exert a beneficial role in terminating inflammation and enhancing tissue repair. Conclusions: We provide histological and transcriptomic-based evidence in humans for the key role of peri-hematomal area in endogenous blood clearance process through the CD163/HO-1 pathway, especially from day 8 after ICH and favored by an anti-inflammatory environment. Our findings contribute to identify innovative therapeutic strategies for ICH.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Cancer Heterogeneity, Plasticity and Resistance to Therapies (CANTHER) - UMR 9020 - UMR 1277
• Lille Neurosciences & Cognition (LilNCog) - U 1172
• Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41

Équipe(s) de recherche :

Genomic @ Lille (GO@L)

Date de dépôt :

2023-12-21T06:53:04Z
2024-01-26T11:27:25Z