Title :

Adherent-Invasive and Non-Invasive Escherichia coli Isolates Differ in Their Effects on Caenorhabditis elegans' Lifespan

Author(s) :

De Sousa Figueiredo, Maria Beatriz [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Pradel, Elizabeth [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
George, Fanny [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Mahieux, Séverine [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Houcke, Isabelle [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Pottier, Muriel [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Fradin, Chantal [Auteur]
Facteurs de risque et déterminants moléculaires des maladies liées au vieillissement (RID-AGE) - U1167
Neut, Christel [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Daniel, Catherine [Auteur]
Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017
Bongiovanni, Antonino [Auteur]
Plateforme BioImaging Center Lille - PLBS [BICeL]
Foligne, Benoit [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Titecat, Marie [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]

Journal title :

Microorganisms

Abbreviated title :

Microorganisms

Volume number :

9

Pages :

1823

Publication date :

2021

ISSN :

2076-2607

English keyword(s) :

adherent-invasive E
coli (AIEC)
Caenorhabditis elegans
gut inflammation
inflammatory bowel disease (IBD)
intestinal epithelial cell

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

The adherent-invasive Escherichia coli (AIEC) pathotype has been implicated in the pathogenesis of inflammatory bowel diseases in general and in Crohn’s disease (CD) in particular. AIEC strains are primarily characterized ...
Show more >The adherent-invasive Escherichia coli (AIEC) pathotype has been implicated in the pathogenesis of inflammatory bowel diseases in general and in Crohn’s disease (CD) in particular. AIEC strains are primarily characterized by their ability to adhere to and invade intestinal epithelial cells. However, the genetic and phenotypic features of AIEC isolates vary greatly as a function of the strain’s clonality, host factors, and the gut microenvironment. It is thus essential to identify the determinants of AIEC pathogenicity and understand their role in intestinal epithelial barrier dysfunction and inflammation. We reasoned that soil nematode Caenorhabditis elegans (a simple but powerful model of host-bacterium interactions) could be used to study the virulence of AIEC vs. non- AIEC E. coli strains. Indeed, we found that the colonization of C. elegans (strain N2) by E. coli impacted survival in a strain-specific manner. Moreover, the AIEC strains’ ability to invade cells in vitro was linked to the median lifespan in C. elegans (strain PX627). However, neither the E. coli intrinsic invasiveness (i.e., the fact for an individual strain to be characterized as invasive or not) nor AIEC’s virulence levels (i.e., the intensity of invasion, established in % from the infectious inoculum) in intestinal epithelial cells was correlated with C. elegans’ lifespan in the killing assay. Nevertheless, AIEC longevity of C. elegans might be a relevant model for screening anti-adhesion drugs and anti-invasive probiotics.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017
• Facteurs de risque et déterminants moléculaires des maladies liées au vieillissement (RID-AGE) - U1167
• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
• Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41

Research team(s) :

Plateforme BioImaging Center Lille (BICeL)

Submission date :

2023-12-21T07:07:29Z
2024-02-23T12:04:41Z
2024-02-23T12:06:41Z