Title :

High-throughput quantitative screening of glucose-stimulated insulin secretion and insulin content using automated MALDI-TOF mass spectrometry

Author(s) :

Delannoy, Clément Philippe [Auteur]
Heuson, Egon [Auteur]
Herledan, Adrien [Auteur]
Oger, Frederik [Auteur]
Thiroux, Bryan [Auteur]
Chevalier, Mickaël [Auteur]
Gromada, Xavier [Auteur]
Rolland, Laure [Auteur]
Froguel, Philippe [Auteur]
Deprez, Benoit [Auteur]
Paul, Sebastien [Auteur]
Annicotte, Jean-Sébastien [Auteur correspondant]
Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 [RID-AGE]
Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 [EGENODIA (GI3M)]

Publication date :

2023-02-15

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Abstract Type 2 diabetes (T2D) is a metabolic disorder characterized by loss of pancreatic β- cell function, decreased insulin secretion and increased insulin resistance, that affects more than 400 million people worldwide. ...
Show more >Abstract Type 2 diabetes (T2D) is a metabolic disorder characterized by loss of pancreatic β- cell function, decreased insulin secretion and increased insulin resistance, that affects more than 400 million people worldwide. Although several treatments are proposed to patients suffering from T2D, long-term control of glycemia remains a challenge. Therefore, identifying new potential drugs and targets that positively affect β-cell function and insulin secretion remains crucial. Here, we developed an automated approach to allow the identification of new compounds or genes potentially involved in β-cell function in a 384-well plate format, using the murine β-cell model Min6. Using MALDI-TOF mass spectrometry, we have implemented a high-throughput screening (HTS) strategy based on the automation of a cellular assay allowing to detect insulin secretion in response to glucose, quantitative detection of insulin, in a miniaturized system. As a proof of concept, we screened siRNA targeting well-know β-cell genes and 1600 chemical compounds and identified several molecules as potential regulators of insulin secretion and/or synthesis, demonstrating that our approach allows HTS of insulin secretion in vitro .Show less >

Language :

Anglais

Collections :

• Facteurs de risque et déterminants moléculaires des maladies liées au vieillissement (RID-AGE) - U1167

Source :

Harvested from HAL