Title :

Transient Receptor Potential Channel Expression Signatures in Tumor-Derived Endothelial Cells: Functional Roles in Prostate Cancer Angiogenesis

Author(s) :

Bernardini, Michela [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Brossa, Alessia [Auteur]
Chinigo, Giorgia [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Grolez, Guillaume [Auteur]
Service de neurologie et pathologie du mouvement
Trimaglio, Giulia [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Allart, Laurent [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Hulot, Audrey [Auteur]
Plateforme de bioinformatique et de biostatistique de Lille - PLBS [Bilille]
Briend, Guillemette [Auteur]
MOdel for Data Analysis and Learning [MODAL]
Genova, Tullio [Auteur]
Joshi, Aditi [Auteur]
Università degli studi di Torino = University of Turin [UNITO]
Mattot, Virginie [Auteur]
Institut Pasteur de Lille
Fromont, Gaëlle [Auteur]
Niche, Nutrition, Cancer et métabolisme oxydatif [N2Cox]
Munaron, Luca [Auteur]
Università degli studi di Torino = University of Turin [UNITO]
Bussolati, Benedetta [Auteur]
Prevarskaya, Natalia [Auteur]
Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate
Fiorio Pla, Alessandra [Auteur]
Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate
Gkika, Dimitra [Auteur]
Rôle des canaux ioniques membranaires et du calcium intracellulaire dans la physiopathologie de la prostate

Journal title :

Cancers

Pages :

956

Publisher :

MDPI

Publication date :

2019-07

ISSN :

2072-6694

HAL domain(s) :

Sciences du Vivant [q-bio]/Cancer

English abstract : [en]

Transient receptor potential (TRP) channels control multiple processes involved in cancer progression by modulating cell proliferation, survival, invasion and intravasation, as well as, endothelial cell (EC) biology and ...
Show more >Transient receptor potential (TRP) channels control multiple processes involved in cancer progression by modulating cell proliferation, survival, invasion and intravasation, as well as, endothelial cell (EC) biology and tumor angiogenesis. Nonetheless, a complete TRP expression signature in tumor vessels, including in prostate cancer (PCa), is still lacking. Methods: In the present study, we profiled by qPCR the expression of all TRP channels in human prostate tumor-derived ECs (TECs) in comparison with TECs from breast and renal tumors. We further functionally characterized the role of the ‘prostate-associated’ channels in proliferation, sprout formation and elongation, directed motility guiding, as well as in vitro and in vivo morphogenesis and angiogenesis. Results: We identified three ‘prostate-associated’ genes whose expression is upregulated in prostate TECs: TRPV2 as a positive modulator of TEC proliferation, TRPC3 as an endothelial PCa cell attraction factor and TRPA1 as a critical TEC angiogenic factor in vitro and in vivo. Conclusions: We provide here the full TRP signature of PCa vascularization among which three play a profound effect on EC biology. These results contribute to explain the aggressive phenotype previously observed in PTEC and provide new putative therapeutic targets.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Laboratoire Paul Painlevé - UMR 8524

Source :

Harvested from HAL