Title :

Postprandial Circulating miRNAs in Response to a Dietary Fat Challenge

Author(s) :

Mantilla-Escalante, Diana [Auteur]
López de las Hazas, María-Carmen [Auteur]
Gil-Zamorano, Judit [Auteur]
del Pozo-Acebo, Lorena [Auteur]
Crespo, M. Carmen [Auteur]
Martín-Hernández, Roberto [Auteur]
del Saz, Andrea [Auteur]
Tomé-Carneiro, Joao [Auteur]
Cardona, Fernando [Auteur]
Cornejo-Pareja, Isabel [Auteur]
García-Ruiz, Almudena [Auteur]
Briand, Olivier [Auteur]
Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U 1011 [RNMCD]
Lasunción, Miguel [Auteur]
Visioli, Francesco [Auteur]
Dávalos, Alberto [Auteur]

Journal title :

Nutrients

Pages :

1326

Publisher :

MDPI

Publication date :

2019-06-13

ISSN :

2072-6643

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Postprandial lipemia has many physiopathological effects, some of which increase the risk of cardiovascular disease. MicroRNAs (miRNAs) can be found in almost all biological fluids, but their postprandial kinetics are ...
Show more >Postprandial lipemia has many physiopathological effects, some of which increase the risk of cardiovascular disease. MicroRNAs (miRNAs) can be found in almost all biological fluids, but their postprandial kinetics are poorly described. We aimed to profile circulating miRNAs in response to a fat challenge. In total, 641 circulating miRNAs were assessed by real-time PCR in plasmas from mice two hours after lipid gavage. Mice with intestine-specific loss of Dicer were screened to identify potential miRNAs released by the intestine. A total of 68 miRNAs were selected for further validation. Ten circulating miRNAs were finally validated as responsive to postprandial lipemia, including miR-206-3p, miR-543-3p, miR-466c-5p, miR-27b-5p, miR-409-3p, miR-340-3p, miR-1941-3p, miR-10a-3p, miR-125a-3p, and miR-468-3p. Analysis of their possible tissues of origin/target showed an enrichment of selected miRNAs in liver, intestine, brain, or skeletal muscle. miR-206, miR-27b-5p, and miR-409-3p were validated in healthy humans. Analysis of their predicted target genes revealed their potential involvement in insulin/insulin like growth factor (insulin/IGF), angiogenesis, cholecystokinin B receptor signaling pathway (CCKR), inflammation or Wnt pathways for mice, and in platelet derived growth factor (PDGF) and CCKR signaling pathways for humans. Therefore, the current study shows that certain miRNAs are released in the circulation in response to fatty meals, proposing them as potential novel therapeutic targets of lipid metabolism.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Récepteurs nucléaires, Maladies Cardiovasculaires et Diabète (RNMCD) - U1011

Source :

Harvested from HAL