A conserved antigen induces respiratory ...
Type de document :
Compte-rendu et recension critique d'ouvrage
PMID :
URL permanente :
Titre :
A conserved antigen induces respiratory Th17-mediated broad serotype protection against pneumococcal superinfection
Auteur(s) :
Liu, Xue [Auteur]
Shenzhen University [Shenzhen]
Université de Lausanne = University of Lausanne [UNIL]
Van Maele, Laurye [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Matarazzo, Laura [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Soulard, Daphnée [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Alves Duarte da Silva, Vinicius [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
de Bakker, Vincent [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Dénéréaz, Julien [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Bock, Florian [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Taschner, Michael [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Ou, Jinzhao [Auteur]
Shenzhen University [Shenzhen]
Gruber, Stephan [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Nizet, Victor [Auteur]
University of California [San Diego] [UC San Diego]
Sirard, Jean-Claude [Auteur correspondant]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Veening, Jan-Willem [Auteur]
University of California [San Diego] [UC San Diego]
Shenzhen University [Shenzhen]
Université de Lausanne = University of Lausanne [UNIL]
Van Maele, Laurye [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Matarazzo, Laura [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Soulard, Daphnée [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Alves Duarte da Silva, Vinicius [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
de Bakker, Vincent [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Dénéréaz, Julien [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Bock, Florian [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Taschner, Michael [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Ou, Jinzhao [Auteur]
Shenzhen University [Shenzhen]
Gruber, Stephan [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Nizet, Victor [Auteur]
University of California [San Diego] [UC San Diego]
Sirard, Jean-Claude [Auteur correspondant]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Veening, Jan-Willem [Auteur]
University of California [San Diego] [UC San Diego]
Titre de la revue :
Cell Host & Microbe
Pagination :
304-314.e8
Date de publication :
2024-03
ISSN :
1931-3128
Mot(s)-clé(s) en anglais :
CRISPRi-seq
Th17
genome-wide vaccinology
intranasal vaccine
non-vaccine serotypes
pneumococcus
protein antigen
superinfection
tissue-resident memory T lymphocytes
vaccine discovery
Th17
genome-wide vaccinology
intranasal vaccine
non-vaccine serotypes
pneumococcus
protein antigen
superinfection
tissue-resident memory T lymphocytes
vaccine discovery
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Several vaccines targeting bacterial pathogens show reduced efficacy upon concurrent viral infection, indicating that a new vaccinology approach is required. To identify antigens for the human pathogen Streptococcus ...
Lire la suite >Several vaccines targeting bacterial pathogens show reduced efficacy upon concurrent viral infection, indicating that a new vaccinology approach is required. To identify antigens for the human pathogen Streptococcus pneumoniae that are effective following influenza infection, we performed CRISPRi-seq in a murine model of superinfection and identified the conserved lafB gene as crucial for virulence. We show that LafB is a membrane-associated, intracellular protein that catalyzes the formation of galactosyl-glucosyl-diacylglycerol, a glycolipid important for cell wall homeostasis. Respiratory vaccination with recombinant LafB, in contrast to subcutaneous vaccination, was highly protective against S. pneumoniae serotypes 2, 15A, and 24F in a murine model. In contrast to standard capsule-based vaccines, protection did not require LafB-specific antibodies but was dependent on airway CD4+ T helper 17 cells. Healthy human individuals can elicit LafB-specific immune responses, indicating LafB antigenicity in humans. Collectively, these findings present a universal pneumococcal vaccine antigen that remains effective following influenza infection.Lire moins >
Lire la suite >Several vaccines targeting bacterial pathogens show reduced efficacy upon concurrent viral infection, indicating that a new vaccinology approach is required. To identify antigens for the human pathogen Streptococcus pneumoniae that are effective following influenza infection, we performed CRISPRi-seq in a murine model of superinfection and identified the conserved lafB gene as crucial for virulence. We show that LafB is a membrane-associated, intracellular protein that catalyzes the formation of galactosyl-glucosyl-diacylglycerol, a glycolipid important for cell wall homeostasis. Respiratory vaccination with recombinant LafB, in contrast to subcutaneous vaccination, was highly protective against S. pneumoniae serotypes 2, 15A, and 24F in a murine model. In contrast to standard capsule-based vaccines, protection did not require LafB-specific antibodies but was dependent on airway CD4+ T helper 17 cells. Healthy human individuals can elicit LafB-specific immune responses, indicating LafB antigenicity in humans. Collectively, these findings present a universal pneumococcal vaccine antigen that remains effective following influenza infection.Lire moins >
Langue :
Anglais
Vulgarisation :
Non
Projet Européen :
Source :
Date de dépôt :
2024-06-11T02:13:36Z