Disruption of tca cycle and glutamate ...
Type de document :
Article dans une revue scientifique: Article original
PMID :
URL permanente :
Titre :
Disruption of tca cycle and glutamate metabolism identified by metabolomics in an in vitro model of amyotrophic lateral sclerosis
Auteur(s) :
Veyrat-Durebex, Charlotte [Auteur]
Corcia, Philippe [Auteur]
Piver, Eric [Auteur]
DEVOS, DAVID [Auteur]
Troubles cognitifs dégénératifs et vasculaires - U1171
Troubles cognitifs dégénératifs et vasculaires - U 1171 - EA 1046 [TCDV]
Troubles cognitifs dégénératifs et vasculaires - U 1171 - EA 1046 [TCDV]
Dangoumau, Audrey [Auteur]
Gouel, Flore [Auteur]
Vourc'h, Patrick [Auteur]
Emond, Patrick [Auteur]
Laumonnier, Frederic [Auteur]
Nadal-Desbarats, Lydie [Auteur]
Gordon, Paul H. [Auteur]
Andres, Christian R. [Auteur]
Blasco, Helene [Auteur]
Corcia, Philippe [Auteur]
Piver, Eric [Auteur]
DEVOS, DAVID [Auteur]
Troubles cognitifs dégénératifs et vasculaires - U1171
Troubles cognitifs dégénératifs et vasculaires - U 1171 - EA 1046 [TCDV]
Troubles cognitifs dégénératifs et vasculaires - U 1171 - EA 1046 [TCDV]
Dangoumau, Audrey [Auteur]
Gouel, Flore [Auteur]
Vourc'h, Patrick [Auteur]
Emond, Patrick [Auteur]
Laumonnier, Frederic [Auteur]
Nadal-Desbarats, Lydie [Auteur]
Gordon, Paul H. [Auteur]
Andres, Christian R. [Auteur]
Blasco, Helene [Auteur]
Titre de la revue :
Molecular neurobiology
Nom court de la revue :
Mol. Neurobiol.
Numéro :
53
Pagination :
6910-6924
Date de publication :
2016-12-01
ISSN :
0893-7648
Mot(s)-clé(s) en anglais :
Metabolomics
Oxidative stress
Co-culture
Astrocytes
GC-MS
Motor neurons
Oxidative stress
Co-culture
Astrocytes
GC-MS
Motor neurons
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
This study aims to develop a cellular metabolomics model that reproduces the pathophysiological conditions found in amyotrophic lateral sclerosis in order to improve knowledge of disease physiology. We used a co-culture ...
Lire la suite >This study aims to develop a cellular metabolomics model that reproduces the pathophysiological conditions found in amyotrophic lateral sclerosis in order to improve knowledge of disease physiology. We used a co-culture model combining the motor neuron-like cell line NSC-34 and the astrocyte clone C8-D1A, with each over-expressing wild-type or G93C mutant human SOD1, to examine amyotrophic lateral sclerosis (ALS) physiology. We focused on the effects of mutant human SOD1 as well as oxidative stress induced by menadione on intracellular metabolism using a metabolomics approach through gas chromatography coupled with mass spectrometry (GC-MS) analysis. Preliminary non-supervised analysis by Principal Component Analysis (PCA) revealed that cell type, genetic environment, and time of culture influenced the metabolomics profiles. Supervised analysis using orthogonal partial least squares discriminant analysis (OPLS-DA) on data from intracellular metabolomics profiles of SOD1G93CLire moins >
Lire la suite >This study aims to develop a cellular metabolomics model that reproduces the pathophysiological conditions found in amyotrophic lateral sclerosis in order to improve knowledge of disease physiology. We used a co-culture model combining the motor neuron-like cell line NSC-34 and the astrocyte clone C8-D1A, with each over-expressing wild-type or G93C mutant human SOD1, to examine amyotrophic lateral sclerosis (ALS) physiology. We focused on the effects of mutant human SOD1 as well as oxidative stress induced by menadione on intracellular metabolism using a metabolomics approach through gas chromatography coupled with mass spectrometry (GC-MS) analysis. Preliminary non-supervised analysis by Principal Component Analysis (PCA) revealed that cell type, genetic environment, and time of culture influenced the metabolomics profiles. Supervised analysis using orthogonal partial least squares discriminant analysis (OPLS-DA) on data from intracellular metabolomics profiles of SOD1G93CLire moins >
Langue :
Anglais
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
CHU Lille
CNRS
Inserm
Université de Lille
CNRS
Inserm
Université de Lille
Collections :
Équipe(s) de recherche :
Troubles cognitifs dégénératifs et vasculaires
Date de dépôt :
2019-11-27T13:38:12Z