Light microscopy morphological characteristics ...
Type de document :
Article dans une revue scientifique: Article original
DOI :
PMID :
URL permanente :
Titre :
Light microscopy morphological characteristics of the sperm flagellum may be related to axonemal abnormalities
Auteur(s) :
Mitchell, Valérie [Auteur]
Sigala, Julien [Auteur]
Ballot, Caroline [Auteur]
Jumeau, F. [Auteur]
Barbotin, Anne-Laure [Auteur]
Duhamel, Alain [Auteur]
Evaluation des technologies de santé et des pratiques médicales - ULR 2694 [METRICS]
Rives, N. [Auteur]
Rigot, J. M. [Auteur]
Escalier, D. [Auteur]
Peers, M. C. [Auteur]
Sigala, Julien [Auteur]
Ballot, Caroline [Auteur]
Jumeau, F. [Auteur]
Barbotin, Anne-Laure [Auteur]
Duhamel, Alain [Auteur]
Evaluation des technologies de santé et des pratiques médicales - ULR 2694 [METRICS]
Rives, N. [Auteur]
Rigot, J. M. [Auteur]
Escalier, D. [Auteur]
Peers, M. C. [Auteur]
Titre de la revue :
Andrologia
Nom court de la revue :
Andrologia
Numéro :
47
Pagination :
214-220
Date de publication :
2015-03-01
ISSN :
0303-4569
Mot(s)-clé(s) en anglais :
electron microscopy
Axoneme
flagellum
light microscopy
sperm motility
Axoneme
flagellum
light microscopy
sperm motility
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Although electron microscopy provides a detailed analysis of ultrastructural abnormalities, this technique is not available in all laboratories. We sought to determine whether certain characteristics of the flagellum as ...
Lire la suite >Although electron microscopy provides a detailed analysis of ultrastructural abnormalities, this technique is not available in all laboratories. We sought to determine whether certain characteristics of the flagellum as assessed by light microscopy were related to axonemal abnormalities. Forty-one patients with an absence of outer dynein arms (type I), a lack of a central complex (type III) and an absence of peripheral doublets (type IV) were studied. Sperm morphology was scored according to David's modified classification. Flagella with an irregular thickness were classified as being of normal length, short or broken. There were correlations between missing outer dynein arms and abnormal, short or coiled flagellum. Type III patients showed the highest flagellar defects (a short (P = 0.0027) or an absent flagellum (P = 0.011)). Just over 68% of the irregular flagella were short in Type III patients, whereas this value was only 34.5% in type I and 26.4% in type IV (P = 0.002). There was a negative correlation between misassembly and spermatozoa of irregular flagella (r = -0.79; P = 0.019). It is concluded that light microscopy analysis of flagellum abnormalities may help provide a correct diagnosis, identify sperm abnormalities with fertility potentials and outcomes in assisted reproduction technologies and assess the genetic risk.Lire moins >
Lire la suite >Although electron microscopy provides a detailed analysis of ultrastructural abnormalities, this technique is not available in all laboratories. We sought to determine whether certain characteristics of the flagellum as assessed by light microscopy were related to axonemal abnormalities. Forty-one patients with an absence of outer dynein arms (type I), a lack of a central complex (type III) and an absence of peripheral doublets (type IV) were studied. Sperm morphology was scored according to David's modified classification. Flagella with an irregular thickness were classified as being of normal length, short or broken. There were correlations between missing outer dynein arms and abnormal, short or coiled flagellum. Type III patients showed the highest flagellar defects (a short (P = 0.0027) or an absent flagellum (P = 0.011)). Just over 68% of the irregular flagella were short in Type III patients, whereas this value was only 34.5% in type I and 26.4% in type IV (P = 0.002). There was a negative correlation between misassembly and spermatozoa of irregular flagella (r = -0.79; P = 0.019). It is concluded that light microscopy analysis of flagellum abnormalities may help provide a correct diagnosis, identify sperm abnormalities with fertility potentials and outcomes in assisted reproduction technologies and assess the genetic risk.Lire moins >
Langue :
Anglais
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
CHU Lille
Université de Lille
Université de Lille
Date de dépôt :
2019-12-09T16:56:13Z