Role of MAdCAM-1-Expressing High Endothelial ...
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Article dans une revue scientifique
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Title :
Role of MAdCAM-1-Expressing High Endothelial Venule-Like Vessels in Colitis Induced in Mice Lacking Sulfotransferases Catalyzing L-Selectin Ligand Biosynthesis
Author(s) :
Low, Shulin [Auteur]
University of Fukui [Bunkyo]
Hirakawa, Jotaro [Auteur]
Chiba University
Hoshino, Hitomi [Auteur]
University of Fukui [Bunkyo]
Uchimura, Kenji [Auteur]
Nagoya University
Kawashima, Hiroto [Auteur]
Chiba University
Kobayashi, Motohiro [Auteur]
University of Fukui [Bunkyo]
University of Fukui [Bunkyo]
Hirakawa, Jotaro [Auteur]
Chiba University
Hoshino, Hitomi [Auteur]
University of Fukui [Bunkyo]
Uchimura, Kenji [Auteur]
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Nagoya University
Kawashima, Hiroto [Auteur]
Chiba University
Kobayashi, Motohiro [Auteur]
University of Fukui [Bunkyo]
Journal title :
The Journal of Histochemistry and Cytochemistry. Official Journal of the Histochemistry Society
Abbreviated title :
J. Histochem. Cytochem.
Volume number :
66
Pages :
415-425
Publication date :
2018-06
ISSN :
1551-5044
English keyword(s) :
inflammatory bowel disease (IBD)
gut-associated lymphoid tissue (GALT)
gut-associated lymphoid tissue (GALT)
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Ulcerative colitis (UC) is a chronic inflammatory disease histologically characterized by diffuse mononuclear cell infiltrates in colonic mucosa. These inflammatory cells are considered to be recruited via high endothelial ...
Show more >Ulcerative colitis (UC) is a chronic inflammatory disease histologically characterized by diffuse mononuclear cell infiltrates in colonic mucosa. These inflammatory cells are considered to be recruited via high endothelial venule (HEV)-like vessels displaying mucosal addressin cell adhesion molecule 1 (MAdCAM-1), the ligand for α4β7 integrin, and/or peripheral lymph node addressin (PNAd), an L-selectin ligand. 6- O-sulfation of N-acetylglucosamine in the carbohydrate moiety of PNAd is catalyzed exclusively by N-acetylglucosamine-6- O-sulfotransferase 1 (GlcNAc6ST-1) and GlcNAc6ST-2. To determine the role of 6- O-sulfation of N-acetylglucosamine on HEV-like vessels in UC, we used a chronic dextran sulfate sodium-induced colitis model using mice deficient in both GlcNAc6ST-1 and GlcNAc6ST-2. We found that more inflammatory cells, with expression of tumor necrosis factor α, were infiltrated in double knockout mouse colitis compared with that in wild-type mice. Moreover, the number of MAdCAM-1-positive vessels was increased in double knockout mouse colitis, and these vessels were bound by E-selectin•IgM chimeras that bind to unsulfated sialyl Lewis X (sLeX). These findings suggest that interactions between MAdCAM-1 and α4β7 integrin and/or unsulfated sLeX and L-selectin may become a dominant mechanism for inflammatory cell recruitment in the absence of 6-sulfo sLeX and contribute to more severe colitis phenotypes seen in double knockout mice.Show less >
Show more >Ulcerative colitis (UC) is a chronic inflammatory disease histologically characterized by diffuse mononuclear cell infiltrates in colonic mucosa. These inflammatory cells are considered to be recruited via high endothelial venule (HEV)-like vessels displaying mucosal addressin cell adhesion molecule 1 (MAdCAM-1), the ligand for α4β7 integrin, and/or peripheral lymph node addressin (PNAd), an L-selectin ligand. 6- O-sulfation of N-acetylglucosamine in the carbohydrate moiety of PNAd is catalyzed exclusively by N-acetylglucosamine-6- O-sulfotransferase 1 (GlcNAc6ST-1) and GlcNAc6ST-2. To determine the role of 6- O-sulfation of N-acetylglucosamine on HEV-like vessels in UC, we used a chronic dextran sulfate sodium-induced colitis model using mice deficient in both GlcNAc6ST-1 and GlcNAc6ST-2. We found that more inflammatory cells, with expression of tumor necrosis factor α, were infiltrated in double knockout mouse colitis compared with that in wild-type mice. Moreover, the number of MAdCAM-1-positive vessels was increased in double knockout mouse colitis, and these vessels were bound by E-selectin•IgM chimeras that bind to unsulfated sialyl Lewis X (sLeX). These findings suggest that interactions between MAdCAM-1 and α4β7 integrin and/or unsulfated sLeX and L-selectin may become a dominant mechanism for inflammatory cell recruitment in the absence of 6-sulfo sLeX and contribute to more severe colitis phenotypes seen in double knockout mice.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
CNRS
Université de Lille
Université de Lille
Collections :
Submission date :
2020-02-12T15:11:33Z
2021-03-19T11:51:47Z
2021-03-19T11:51:47Z
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