Actively detached Pseudomonas aeruginosa ...
Document type :
Article dans une revue scientifique: Article original
Permalink :
Title :
Actively detached Pseudomonas aeruginosa biofilm cell susceptibility to benzalkonium chloride and associated resistance mechanism
Author(s) :
Khelissa, Simon [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Abdallah, Marwan [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Jama, charafeddine [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Chihib, Nour-Eddine [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Unité Matériaux et Transformations - UMR 8207 [UMET]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Abdallah, Marwan [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Jama, charafeddine [Auteur]

Unité Matériaux et Transformations - UMR 8207 [UMET]
Chihib, Nour-Eddine [Auteur]

Unité Matériaux et Transformations (UMET) - UMR 8207
Unité Matériaux et Transformations - UMR 8207 [UMET]
Journal title :
Archives of Microbiology
Abbreviated title :
Arch Microbiol
Volume number :
201
Pages :
747-755
Publisher :
Springer Science and Business Media LLC
Publication date :
2019-03-05
HAL domain(s) :
Chimie/Polymères
Chimie/Matériaux
Sciences du Vivant [q-bio]
Chimie/Matériaux
Sciences du Vivant [q-bio]
English abstract : [en]
The present work aimed at studying physiological properties of Pseudomonas aeruginosa cells actively detached from biofilm formed on stainless steel and comparing them with their planktonic counterparts as a function of ...
Show more >The present work aimed at studying physiological properties of Pseudomonas aeruginosa cells actively detached from biofilm formed on stainless steel and comparing them with their planktonic counterparts as a function of growth temperature (20 °C and 37 °C). The susceptibility of P. aeruginosa cells to benzalkonium chloride (BAC) was studied. Furthermore, the effect of BAC on the cell membrane integrity and the role of the cell membrane fluidity in the cell-scale-resistance mechanism were investigated. Our results showed that actively detached biofilm cells were more susceptible to BAC treatment than planktonic ones. A greater leakage of intracellular potassium after BAC addition was observed in actively detached biofilm cells, which reflects their membrane vulnerability. The rise of the growth temperature from 20 to 37 °C increased the membrane rigidity of planktonic cells comparatively to their actively detached biofilm ones. Under experimental conditions developed in this work, our data highlighted that actively biofilm-detached and planktonic P. aeruginosa cells have distinguishable phenotypes.Show less >
Show more >The present work aimed at studying physiological properties of Pseudomonas aeruginosa cells actively detached from biofilm formed on stainless steel and comparing them with their planktonic counterparts as a function of growth temperature (20 °C and 37 °C). The susceptibility of P. aeruginosa cells to benzalkonium chloride (BAC) was studied. Furthermore, the effect of BAC on the cell membrane integrity and the role of the cell membrane fluidity in the cell-scale-resistance mechanism were investigated. Our results showed that actively detached biofilm cells were more susceptible to BAC treatment than planktonic ones. A greater leakage of intracellular potassium after BAC addition was observed in actively detached biofilm cells, which reflects their membrane vulnerability. The rise of the growth temperature from 20 to 37 °C increased the membrane rigidity of planktonic cells comparatively to their actively detached biofilm ones. Under experimental conditions developed in this work, our data highlighted that actively biofilm-detached and planktonic P. aeruginosa cells have distinguishable phenotypes.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
CNRS
INRA
ENSCL
CNRS
INRA
ENSCL
Collections :
Research team(s) :
Ingénierie des Systèmes Polymères
Processus aux Interfaces et Hygiène des Matériaux (PIHM)
Processus aux Interfaces et Hygiène des Matériaux (PIHM)
Submission date :
2020-07-09T17:19:13Z
2020-08-27T13:06:19Z
2021-05-25T12:20:53Z
2020-08-27T13:06:19Z
2021-05-25T12:20:53Z