Omega-3 fatty acid-derived oxylipins reduce ...
Document type :
Communication dans un congrès avec actes: Autre communication scientifique (congrès sans actes - poster - séminaire...)
Title :
Omega-3 fatty acid-derived oxylipins reduce inflammation response in human macrophages: putative mechanism through PPAR-gamma binding
Author(s) :
Bosviel, Rémy [Auteur]
Unité de Nutrition Humaine [UNH]
Université d'Auvergne - Clermont-Ferrand I [UdA]
Joumard-Cubizolles, Laurie [Auteur]
Unité de Nutrition Humaine [UNH]
Chinetti-Gbaguidi, Giulia [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Université de Lille
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Institut de Recherche sur le Cancer et le Vieillissement [IRCAN]
Université de Nice Sophia-Antipolis [UNSA]
Centre National de la Recherche Scientifique [CNRS]
Bayle, Dominique [Auteur]
Unité de Nutrition Humaine [UNH]
Copin, Corinne [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Université de Lille
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Hennuyer, Nathalie [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Université de Lille
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Staels, Bart [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Université de Lille
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Zanoni, Giuseppe [Auteur]
Università degli Studi di Pavia = University of Pavia [UNIPV]
Porta, A. [Auteur]
Università degli Studi di Pavia = University of Pavia [UNIPV]
Balas, Laurence [Auteur]
Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]
Centre National de la Recherche Scientifique [CNRS]
Université de Montpellier [UM]
Ecole Nationale Supérieure de Chimie de Montpellier [ENSCM]
Galano, Jean-Marie [Auteur]
Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]
Centre National de la Recherche Scientifique [CNRS]
Université de Montpellier [UM]
Ecole Nationale Supérieure de Chimie de Montpellier [ENSCM]
Oger, Camille [Auteur]
Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]
Centre National de la Recherche Scientifique [CNRS]
Université de Montpellier [UM]
Ecole Nationale Supérieure de Chimie de Montpellier [ENSCM]
Mazur, André [Auteur]
Unité de Nutrition Humaine [UNH]
Durand, Thierry [Auteur]
Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]
Centre National de la Recherche Scientifique [CNRS]
Université de Montpellier [UM]
Ecole Nationale Supérieure de Chimie de Montpellier [ENSCM]
Gladine, Cécile [Orateur]
Unité de Nutrition Humaine [UNH]
Unité de Nutrition Humaine [UNH]
Université d'Auvergne - Clermont-Ferrand I [UdA]
Joumard-Cubizolles, Laurie [Auteur]
Unité de Nutrition Humaine [UNH]
Chinetti-Gbaguidi, Giulia [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Université de Lille
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Institut de Recherche sur le Cancer et le Vieillissement [IRCAN]
Université de Nice Sophia-Antipolis [UNSA]
Centre National de la Recherche Scientifique [CNRS]
Bayle, Dominique [Auteur]
Unité de Nutrition Humaine [UNH]
Copin, Corinne [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Université de Lille
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Hennuyer, Nathalie [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Université de Lille
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Staels, Bart [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Université de Lille
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Zanoni, Giuseppe [Auteur]
Università degli Studi di Pavia = University of Pavia [UNIPV]
Porta, A. [Auteur]
Università degli Studi di Pavia = University of Pavia [UNIPV]
Balas, Laurence [Auteur]
Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]
Centre National de la Recherche Scientifique [CNRS]
Université de Montpellier [UM]
Ecole Nationale Supérieure de Chimie de Montpellier [ENSCM]
Galano, Jean-Marie [Auteur]
Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]
Centre National de la Recherche Scientifique [CNRS]
Université de Montpellier [UM]
Ecole Nationale Supérieure de Chimie de Montpellier [ENSCM]
Oger, Camille [Auteur]
Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]
Centre National de la Recherche Scientifique [CNRS]
Université de Montpellier [UM]
Ecole Nationale Supérieure de Chimie de Montpellier [ENSCM]
Mazur, André [Auteur]
Unité de Nutrition Humaine [UNH]
Durand, Thierry [Auteur]
Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] [IBMM]
Centre National de la Recherche Scientifique [CNRS]
Université de Montpellier [UM]
Ecole Nationale Supérieure de Chimie de Montpellier [ENSCM]
Gladine, Cécile [Orateur]
Unité de Nutrition Humaine [UNH]
Conference title :
6. European Workshop on Lipid Mediators (6EWLM)
Conference organizers(s) :
ESLM.
Goethe-Universität Frankfurt am Main. DEU.
Collaborative Research Centre 1039.
Goethe-Universität Frankfurt am Main. DEU.
Collaborative Research Centre 1039.
City :
Frankfurt
Country :
Allemagne
Start date of the conference :
2016-09-27
Journal title :
6th European Workshop on Lipid Mediators (6EWLM) Book of Abstracts
Publication date :
2016
Keyword(s) :
oxylipine
acide gras oméga 3
propriété anti-inflammatoire
mécanisme d'action
acide gras oméga 3
propriété anti-inflammatoire
mécanisme d'action
HAL domain(s) :
Sciences du Vivant [q-bio]/Alimentation et Nutrition
English abstract : [en]
The anti-inflammatory properties of omega 3 fatty acids have been largely demonstrated in vitro and in vivo but research gaps remain regarding the contribution of their oxygenated metabolites also called oxylipins. We aimed ...
Show more >The anti-inflammatory properties of omega 3 fatty acids have been largely demonstrated in vitro and in vivo but research gaps remain regarding the contribution of their oxygenated metabolites also called oxylipins. We aimed to investigate and compare the anti-inflammatory properties and potential mechanisms of action of different types of omega 3 fatty acid-derived oxylipins including (i) four DHA-derived oxylipins, i.e. Neuroprostanes (14-A4- and 4(RS)-4-F4t-NeuroP), Protectin DX (PDX) as well as Neuroprotectin D1 (NPD1/PD1), (ii) a n-3 DPA derived oxylipin i.e. 10S,17S-diH n-3 DPAEEZ, (iii) two phytoprostanes (16-B1-PhytoP and 9-L1-PhytoP) and their enantiomers and one phytofuran (ent-16-(RS)-epi-9-PhytoF).Human peripheral blood mononuclear cells were isolated from healthy donors by Ficoll density gradient centrifugation. Monocytes were differentiated into resting macrophages (RM) for 7 days. RM were exposed to the different types of oxylipins at 3 different doses (i.e. 0.1, 1 and 10 μM) during 30 min. The inflammatory response was then induced with LPS (100 ng/mL) for 6 hours.Preliminary results of gene expression analysis (qPCR) show that IL-6, MCP-1, COX-2, TNF-alpha or CCL3 mRNA were significantly lower in macrophages pre-exposed to 10μM 14-A4-NeuroP (-84%, -57%, -29%, -41% and -23% respectively). Significant but less pronounced effects on IL-6 and MCP-1 were also observed with 10μM 4(RS)-4-F4t-NeuroP (-25% and -25% respectively). Reduced levels of TNF-alpha protein secretion (ELISA) were found in macrophages pre-exposed to 10μM 4(RS)-4-F4t-NeuroP (-12% p<0.05) while measurable but less pronounced effects were observed with 14-A4-NeuroP, PDX, PD1 or 10S,17S-diH n-3 DPAEEZ (-9%, -22%, -10% and -15% ns, respectively). Abundance and phosphorylation of IκB-alpha (Western Blot) suggest that 14-A4- and 4(RS)-4-F4t-NeuroPs could exert their anti-inflammatory effects through the inhibition of IκB-alpha phosphorylation. Finally, cotransfection of luciferase reporter vector with human PPAR-gamma expression vector performed in Cos-7 cells suggests that all tested oxylipins may act in part through PPAR-gamma.In conclusion, these results suggest that the anti-inflammatory properties of omega 3 fatty acids could be mediated, at least in part, by oxylipins, and bring new insights into their mechanism of actionShow less >
Show more >The anti-inflammatory properties of omega 3 fatty acids have been largely demonstrated in vitro and in vivo but research gaps remain regarding the contribution of their oxygenated metabolites also called oxylipins. We aimed to investigate and compare the anti-inflammatory properties and potential mechanisms of action of different types of omega 3 fatty acid-derived oxylipins including (i) four DHA-derived oxylipins, i.e. Neuroprostanes (14-A4- and 4(RS)-4-F4t-NeuroP), Protectin DX (PDX) as well as Neuroprotectin D1 (NPD1/PD1), (ii) a n-3 DPA derived oxylipin i.e. 10S,17S-diH n-3 DPAEEZ, (iii) two phytoprostanes (16-B1-PhytoP and 9-L1-PhytoP) and their enantiomers and one phytofuran (ent-16-(RS)-epi-9-PhytoF).Human peripheral blood mononuclear cells were isolated from healthy donors by Ficoll density gradient centrifugation. Monocytes were differentiated into resting macrophages (RM) for 7 days. RM were exposed to the different types of oxylipins at 3 different doses (i.e. 0.1, 1 and 10 μM) during 30 min. The inflammatory response was then induced with LPS (100 ng/mL) for 6 hours.Preliminary results of gene expression analysis (qPCR) show that IL-6, MCP-1, COX-2, TNF-alpha or CCL3 mRNA were significantly lower in macrophages pre-exposed to 10μM 14-A4-NeuroP (-84%, -57%, -29%, -41% and -23% respectively). Significant but less pronounced effects on IL-6 and MCP-1 were also observed with 10μM 4(RS)-4-F4t-NeuroP (-25% and -25% respectively). Reduced levels of TNF-alpha protein secretion (ELISA) were found in macrophages pre-exposed to 10μM 4(RS)-4-F4t-NeuroP (-12% p<0.05) while measurable but less pronounced effects were observed with 14-A4-NeuroP, PDX, PD1 or 10S,17S-diH n-3 DPAEEZ (-9%, -22%, -10% and -15% ns, respectively). Abundance and phosphorylation of IκB-alpha (Western Blot) suggest that 14-A4- and 4(RS)-4-F4t-NeuroPs could exert their anti-inflammatory effects through the inhibition of IκB-alpha phosphorylation. Finally, cotransfection of luciferase reporter vector with human PPAR-gamma expression vector performed in Cos-7 cells suggests that all tested oxylipins may act in part through PPAR-gamma.In conclusion, these results suggest that the anti-inflammatory properties of omega 3 fatty acids could be mediated, at least in part, by oxylipins, and bring new insights into their mechanism of actionShow less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Non spécifiée
Popular science :
Non
Source :