Title :

Decrease of IL-5 production by naive T cells cocultured with IL-18-producing BCG-pulsed dendritic cells from patients allergic to house dust mite

Author(s) :

Kowalewicz-Kulbat, Magdalena [Auteur correspondant]
University of Lódź
Szpakowski, Piotr [Auteur]
Medical University of Łódź [MUL]
University of Lódź
Krawczyk, Krzysztof [Auteur]
University of Lódź
Kowalski, Marek [Auteur]
Medical University of Łódź [MUL]
Kosinski, Slawomir [Auteur]
Medical University of Łódź [MUL]
Biet, Franck [Auteur]
Infectiologie et Santé Publique [UMR ISP]
Rudnicka, Wieslawa [Auteur]
University of Lódź
Locht, Camille [Auteur]
University of Lódź
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]

Journal title :

Vaccines

Pages :

14 p.

Publisher :

MDPI

Publication date :

2021-03-18

ISSN :

2076-393X

English keyword(s) :

BCG
Der p 1
Th1/Th2 cells
asthma
dendritic cells
rBCG-hIL-18

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

The only currently available anti-tuberculosis vaccine, Bacillus Calmette-Guérin (BCG), has been reported to also protect against unrelated diseases, including inflammatory diseases such as allergic asthma. Recombinant BCG ...
Show more >The only currently available anti-tuberculosis vaccine, Bacillus Calmette-Guérin (BCG), has been reported to also protect against unrelated diseases, including inflammatory diseases such as allergic asthma. Recombinant BCG strains that produce IL-18 have been shown to enhance Th1 responses over non-recombinant BCG and to reduce IL-5 production and bronchoalveolar eosinophilia in mice. However, their ability to decrease the immune polarization of human Th2 cells is not known. Here, we show that BCG and recombinant BCG producing human IL-18 (rBCG-hIL-18) induced the maturation of Der p 1-stimulated monocyte-derived dendritic cells (MD-DCs) from healthy controls and from patients allergic to house dust mites. After incubation with mycobacteria and Der p 1, MD-DCs produced significantly more IL-23 and IP-10 but had no effect on IL-12p70 or IL-10 production compared to Der p 1-pulsed MD-DCs in the absence of mycobacteria. In the presence of Der p 1, BCG- and rBCG-hIL-18-pulsed MD-DCs cocultured with naive, but not with memory T cells from allergic patients, resulted in a decrease in IL-5 production compared to non-pulsed MD-DCs cultured in the presence of Der p 1. BCG, and especially rBCG-hIL-18, may thus be potential therapeutic tools to reduce exacerbated Th2 responses in patients with allergic asthma.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL