Identification of Post-translational ...
Type de document :
Compte-rendu et recension critique d'ouvrage
DOI :
Titre :
Identification of Post-translational Modifications on Odorant-Binding Protein Isoforms from Pig Olfactory Secretome by High-Resolution Mass Spectrometry: O-β-N-acetylglucosaminylation and Phosphorylation
Auteur(s) :
Bouclon, Julien [Auteur]
Laboratoire des biomolécules [LBM UMR 7203]
Le Danvic, Chrystelle [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Guettier, Elodie [Auteur]
Unité Expérimentale de Physiologie Animale de l‘Orfrasiére [UE PAO]
Bray, Fabrice [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Tokarski, Caroline [Auteur]
Centre National de la Recherche Scientifique [CNRS]
Rolando, Christian [Auteur]
Laboratoire de Chimie Organique et Macromoleculaire [UMR CNRS 8009]
Nagnan-Le Meillour, Patricia [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Laboratoire des biomolécules [LBM UMR 7203]
Le Danvic, Chrystelle [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Guettier, Elodie [Auteur]
Unité Expérimentale de Physiologie Animale de l‘Orfrasiére [UE PAO]
Bray, Fabrice [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Tokarski, Caroline [Auteur]
Centre National de la Recherche Scientifique [CNRS]
Rolando, Christian [Auteur]
Laboratoire de Chimie Organique et Macromoleculaire [UMR CNRS 8009]
Nagnan-Le Meillour, Patricia [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Titre de la revue :
Frontiers in Ecology and Evolution
Pagination :
80-85
Éditeur :
Frontiers Media
Date de publication :
2017-11-22
ISSN :
2296-701X
Discipline(s) HAL :
Sciences du Vivant [q-bio]/Biologie cellulaire
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
Résumé en anglais : [en]
Odorant-Binding Proteins (OBP) are major players of perireceptor events in olfaction. Despite their importance, a molecular mechanism explaining their specificity for odors and pheromones has yet to be proposed. A new ...
Lire la suite >Odorant-Binding Proteins (OBP) are major players of perireceptor events in olfaction. Despite their importance, a molecular mechanism explaining their specificity for odors and pheromones has yet to be proposed. A new approach is provided by the analysis of the pig olfactory secretome that is mainly composed of OBP isoforms, generated from 3 gene products by two types of post-translational modifications (PTM): (i) phosphorylation and (ii) O-β-N-acetylglucosaminylation (O-GlcNAcylation), which are unusual for secreted proteins. Although both types of PTM can be demonstrated on OBP isoforms by specific antibodies, they have to be identified by mass spectrometry (MS), as localizing PTM sites and identifying PTM patterns can help predict binding affinities. In this paper, we report the identification of phosphorylation and O-GlcNAcylation sites on peptides coming from trypsin digestion of only OBP (sensu stricto) by nanoLC-nanoESI-HCD-MS/MS-Orbitrap. These PTM were not present on VEG and SAL. PEAKS software analysis of raw MS data allowed selecting spectra that were analyzed manually to identify PTMs. Four peptides corresponding to two different portions of OBP sequence were modified either by a phosphate group or by a hexNAc moiety. Due to the high energy used in HCD, the data did not allow precise localization of the modified sites. The new findings contribute to a better understanding of the mechanisms by which OBP isoforms could extend the binding repertoire of the secreted OBPs. Data are available via ProteomeXchange with identifier PXD007955.Lire moins >
Lire la suite >Odorant-Binding Proteins (OBP) are major players of perireceptor events in olfaction. Despite their importance, a molecular mechanism explaining their specificity for odors and pheromones has yet to be proposed. A new approach is provided by the analysis of the pig olfactory secretome that is mainly composed of OBP isoforms, generated from 3 gene products by two types of post-translational modifications (PTM): (i) phosphorylation and (ii) O-β-N-acetylglucosaminylation (O-GlcNAcylation), which are unusual for secreted proteins. Although both types of PTM can be demonstrated on OBP isoforms by specific antibodies, they have to be identified by mass spectrometry (MS), as localizing PTM sites and identifying PTM patterns can help predict binding affinities. In this paper, we report the identification of phosphorylation and O-GlcNAcylation sites on peptides coming from trypsin digestion of only OBP (sensu stricto) by nanoLC-nanoESI-HCD-MS/MS-Orbitrap. These PTM were not present on VEG and SAL. PEAKS software analysis of raw MS data allowed selecting spectra that were analyzed manually to identify PTMs. Four peptides corresponding to two different portions of OBP sequence were modified either by a phosphate group or by a hexNAc moiety. Due to the high energy used in HCD, the data did not allow precise localization of the modified sites. The new findings contribute to a better understanding of the mechanisms by which OBP isoforms could extend the binding repertoire of the secreted OBPs. Data are available via ProteomeXchange with identifier PXD007955.Lire moins >
Langue :
Anglais
Vulgarisation :
Non
Source :
Fichiers
- https://www.frontiersin.org/articles/10.3389/fevo.2017.00142/pdf
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