Surface Functionalization with Polyethylene ...
Type de document :
Compte-rendu et recension critique d'ouvrage
DOI :
Titre :
Surface Functionalization with Polyethylene Glycol and Polyethyleneimine Improves the Performance of Graphene-Based Materials for Safe and Efficient Intracellular Delivery by Laser-Induced Photoporation
Auteur(s) :
Liu, Jing [Auteur]
Li, Chengnan [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Brans, Toon [Auteur]
Harizaj, Aranit [Auteur]
van de Steene, Shana [Auteur]
de Beer, Thomas [Auteur]
de Smedt, Stefaan [Auteur]
Nanjing Forestry University [NFU]
Szunerits, Sabine [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Boukherroub, Rabah [Auteur]
NanoBioInterfaces - IEMN [NBI - IEMN]
Xiong, Ranhua [Auteur]
Braeckmans, Kevin [Auteur]
Li, Chengnan [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Brans, Toon [Auteur]
Harizaj, Aranit [Auteur]
van de Steene, Shana [Auteur]
de Beer, Thomas [Auteur]
de Smedt, Stefaan [Auteur]
Nanjing Forestry University [NFU]
Szunerits, Sabine [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Boukherroub, Rabah [Auteur]
NanoBioInterfaces - IEMN [NBI - IEMN]
Xiong, Ranhua [Auteur]
Braeckmans, Kevin [Auteur]
Titre de la revue :
International Journal of Molecular Sciences
Pagination :
1540
Éditeur :
MDPI
Date de publication :
2020
ISSN :
1661-6596
Mot(s)-clé(s) en anglais :
photoporation
intracellular delivery
nanomaterial functionalization
graphene-based materials
colloidal stability
intracellular delivery
nanomaterial functionalization
graphene-based materials
colloidal stability
Discipline(s) HAL :
Chimie/Matériaux
Chimie/Polymères
Sciences du Vivant [q-bio]/Sciences pharmaceutiques/Pharmacie galénique
Chimie/Polymères
Sciences du Vivant [q-bio]/Sciences pharmaceutiques/Pharmacie galénique
Résumé en anglais : [en]
Nanoparticle mediated laser-induced photoporation is a physical cell membrane disruption approach to directly deliver extrinsic molecules into living cells, which is particularly promising in applications for both adherent ...
Lire la suite >Nanoparticle mediated laser-induced photoporation is a physical cell membrane disruption approach to directly deliver extrinsic molecules into living cells, which is particularly promising in applications for both adherent and suspension cells. In this work, we explored surface modifications of graphene quantum dots (GQD) and reduced graphene oxide (rGO) with polyethylene glycol (PEG) and polyethyleneimine (PEI) to enhance colloidal stability while retaining photoporation functionality. After photoporation with FITC-dextran 10 kDa (FD10), the percentage of positive HeLa cells (81% for GQD-PEG, 74% for rGO-PEG and 90% for rGO-PEI) increased approximately two-fold compared to the bare nanomaterials. While for Jurkat suspension cells, the photoporationefficiency with polymer-modified graphene-based nanomaterial reached as high as 80%. Cell viability was >80% in all these cases. In addition, polymer functionalization proved to be beneficial for the delivery of larger macromolecules (FD70 and FD500) as well. Finally, we show that rGO is suitable for photoporation using a near-infrared laser to reach 80% FD10 positive HeLa cells at 80% cell viability. We conclude that modification of graphene-based nanoparticles with PEG and especially PEI provide better colloidal stability in cell medium, resulting in more uniform transfection and overall increased efficiencyLire moins >
Lire la suite >Nanoparticle mediated laser-induced photoporation is a physical cell membrane disruption approach to directly deliver extrinsic molecules into living cells, which is particularly promising in applications for both adherent and suspension cells. In this work, we explored surface modifications of graphene quantum dots (GQD) and reduced graphene oxide (rGO) with polyethylene glycol (PEG) and polyethyleneimine (PEI) to enhance colloidal stability while retaining photoporation functionality. After photoporation with FITC-dextran 10 kDa (FD10), the percentage of positive HeLa cells (81% for GQD-PEG, 74% for rGO-PEG and 90% for rGO-PEI) increased approximately two-fold compared to the bare nanomaterials. While for Jurkat suspension cells, the photoporationefficiency with polymer-modified graphene-based nanomaterial reached as high as 80%. Cell viability was >80% in all these cases. In addition, polymer functionalization proved to be beneficial for the delivery of larger macromolecules (FD70 and FD500) as well. Finally, we show that rGO is suitable for photoporation using a near-infrared laser to reach 80% FD10 positive HeLa cells at 80% cell viability. We conclude that modification of graphene-based nanoparticles with PEG and especially PEI provide better colloidal stability in cell medium, resulting in more uniform transfection and overall increased efficiencyLire moins >
Langue :
Anglais
Vulgarisation :
Non
Source :
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- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073198/pdf
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- https://hal.archives-ouvertes.fr/hal-03090057/document
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- ijms-21-01540.pdf
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