High spatial resolution electrochemical ...
Type de document :
Compte-rendu et recension critique d'ouvrage
Titre :
High spatial resolution electrochemical biosensing using reflected light microscopy
Auteur(s) :
Munteanu, Raluca-Elena [Auteur]
Ye, Ran [Auteur]
Université Catholique de Louvain = Catholic University of Louvain [UCL]
Polonschii, Cristina [Auteur]
Ruff, Adrian [Auteur]
Gheorghiu, Mihaela [Auteur]
Gheorghiu, Eugen [Auteur]
Boukherroub, Rabah [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
NanoBioInterfaces - IEMN [NBI - IEMN]
Schuhmann, Wolfgang [Auteur]
Melinte, Sorin [Auteur]
Université Catholique de Louvain = Catholic University of Louvain [UCL]
Gaspar, Szilveszter [Auteur]
Ye, Ran [Auteur]
Université Catholique de Louvain = Catholic University of Louvain [UCL]
Polonschii, Cristina [Auteur]
Ruff, Adrian [Auteur]
Gheorghiu, Mihaela [Auteur]
Gheorghiu, Eugen [Auteur]
Boukherroub, Rabah [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
NanoBioInterfaces - IEMN [NBI - IEMN]
Schuhmann, Wolfgang [Auteur]
Melinte, Sorin [Auteur]
Université Catholique de Louvain = Catholic University of Louvain [UCL]
Gaspar, Szilveszter [Auteur]
Titre de la revue :
Scientific Reports
Éditeur :
Nature Publishing Group
Date de publication :
2019-10-23
ISSN :
2045-2322
Mot(s)-clé(s) en anglais :
Analytical chemistry
Bioanalytical chemistry
Electrochemistry
Sensors
Techniques and instrumentation
Bioanalytical chemistry
Electrochemistry
Sensors
Techniques and instrumentation
Discipline(s) HAL :
Chimie
Chimie/Chimie analytique
Chimie/Chimie analytique
Résumé en anglais : [en]
If the analyte does not only change the electrochemical but also the optical properties of the electrode/solution interface, the spatial resolution of an electrochemical sensor can be substantially enhanced by combining ...
Lire la suite >If the analyte does not only change the electrochemical but also the optical properties of the electrode/solution interface, the spatial resolution of an electrochemical sensor can be substantially enhanced by combining the electrochemical sensor with optical microscopy. In order to demonstrate this, electrochemical biosensors for the detection of hydrogen peroxide and glucose were developed by drop casting enzyme and redox polymer mixtures onto planar, optically transparent electrodes. These biosensors generate current signals proportional to the analyte concentration via a reaction sequence which ultimately changes the oxidation state of the redox polymer. Images of the interface of these biosensors were acquired using bright field reflected light microscopy (BFRLM). Analysis showed that the intensity of these images is higher when the redox polymer is oxidized than when it is reduced. It also revealed that the time needed for the redox polymer to change oxidation state can be assayed optically and is dependent on the concentration of the analyte. By combining the biosensor for hydrogen peroxide detection with BFRLM, it was possible to determine hydrogen peroxide in concentrations as low as 12.5 µM with a spatial resolution of 12 µm × 12 µm, without the need for the fabrication of microelectrodes of these dimensions.Lire moins >
Lire la suite >If the analyte does not only change the electrochemical but also the optical properties of the electrode/solution interface, the spatial resolution of an electrochemical sensor can be substantially enhanced by combining the electrochemical sensor with optical microscopy. In order to demonstrate this, electrochemical biosensors for the detection of hydrogen peroxide and glucose were developed by drop casting enzyme and redox polymer mixtures onto planar, optically transparent electrodes. These biosensors generate current signals proportional to the analyte concentration via a reaction sequence which ultimately changes the oxidation state of the redox polymer. Images of the interface of these biosensors were acquired using bright field reflected light microscopy (BFRLM). Analysis showed that the intensity of these images is higher when the redox polymer is oxidized than when it is reduced. It also revealed that the time needed for the redox polymer to change oxidation state can be assayed optically and is dependent on the concentration of the analyte. By combining the biosensor for hydrogen peroxide detection with BFRLM, it was possible to determine hydrogen peroxide in concentrations as low as 12.5 µM with a spatial resolution of 12 µm × 12 µm, without the need for the fabrication of microelectrodes of these dimensions.Lire moins >
Langue :
Anglais
Vulgarisation :
Non
Source :
Fichiers
- https://www.nature.com/articles/s41598-019-50949-9.pdf
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- https://hal.archives-ouvertes.fr/hal-02367965/document
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- https://hal.archives-ouvertes.fr/hal-02367965/document
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- Munteanu_2019_s41598-019-50949-9.pdf
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