Method for Macromolecular Colocalization ...
Type de document :
Compte-rendu et recension critique d'ouvrage
DOI :
Titre :
Method for Macromolecular Colocalization Using Atomic Recombination in Dynamic SIMS
Auteur(s) :
Legent, G. [Auteur]
Delaune, A. [Auteur]
Norris, V. [Auteur]
Université de Rouen Normandie [UNIROUEN]
Delcorte, A. [Auteur]
Institut de la matière condensée et des nanosciences / Institute of Condensed Matter and Nanosciences [IMCN]
Gibouin, David [Auteur]
Université de Rouen Normandie [UNIROUEN]
Lefebvre, Fabrice [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Misevic, G. [Auteur]
Thellier, M. [Auteur]
CHU Pitié-Salpêtrière [AP-HP]
Ripoll, C. [Auteur]
Université de Rouen Normandie [UNIROUEN]
Delaune, A. [Auteur]
Norris, V. [Auteur]
Université de Rouen Normandie [UNIROUEN]
Delcorte, A. [Auteur]
Institut de la matière condensée et des nanosciences / Institute of Condensed Matter and Nanosciences [IMCN]
Gibouin, David [Auteur]
Université de Rouen Normandie [UNIROUEN]
Lefebvre, Fabrice [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Misevic, G. [Auteur]
Thellier, M. [Auteur]
CHU Pitié-Salpêtrière [AP-HP]
Ripoll, C. [Auteur]
Université de Rouen Normandie [UNIROUEN]
Titre de la revue :
Journal of Physical Chemistry B
Pagination :
5534-5546
Éditeur :
American Chemical Society
Date de publication :
2008-05
ISSN :
1520-6106
Discipline(s) HAL :
Sciences de l'ingénieur [physics]/Matériaux
Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Localizing two or more components of assemblies in biological systems requires both continued development of fluorescence techniques and invention of entirely new techniques. Candidates for the latter include dynamic ...
Lire la suite >Localizing two or more components of assemblies in biological systems requires both continued development of fluorescence techniques and invention of entirely new techniques. Candidates for the latter include dynamic secondary ion mass spectrometry (D-SIMS). The latest generation of D-SIMS, the Cameca NanoSIMS 50, permits the localization of specific, isotopically labeled molecules and macromolecules in sections of biological material with a resolution in the tens of nanometers and with a sensitivity approaching in principle that of a single protein. Here we use two different systems, crystals of glycine and mixtures of proteins, to show that the formation of recombinant CN secondary ions under Cs bombardment can be exploited to create a new colocalization technique. We show experimentally that the formation of the recombinant 13C15N secondary ion between 13C- and 15N-labeled macromolecules is indeed an indicator of the distance between the interacting macromolecules and on their shape. We build up a convolution model of the mixing-recombination process in D-SIMS that allows quantitative interpretations of the distance-dependent formation of the recombinant CN. Our results show that macromolecules can be colocalized if they are within 2 nm of one another. We discuss the potential advantages of this new technique for biological applications.Lire moins >
Lire la suite >Localizing two or more components of assemblies in biological systems requires both continued development of fluorescence techniques and invention of entirely new techniques. Candidates for the latter include dynamic secondary ion mass spectrometry (D-SIMS). The latest generation of D-SIMS, the Cameca NanoSIMS 50, permits the localization of specific, isotopically labeled molecules and macromolecules in sections of biological material with a resolution in the tens of nanometers and with a sensitivity approaching in principle that of a single protein. Here we use two different systems, crystals of glycine and mixtures of proteins, to show that the formation of recombinant CN secondary ions under Cs bombardment can be exploited to create a new colocalization technique. We show experimentally that the formation of the recombinant 13C15N secondary ion between 13C- and 15N-labeled macromolecules is indeed an indicator of the distance between the interacting macromolecules and on their shape. We build up a convolution model of the mixing-recombination process in D-SIMS that allows quantitative interpretations of the distance-dependent formation of the recombinant CN. Our results show that macromolecules can be colocalized if they are within 2 nm of one another. We discuss the potential advantages of this new technique for biological applications.Lire moins >
Langue :
Anglais
Vulgarisation :
Non
Source :
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