Simultaneous genotyping of snails and ...
Type de document :
Compte-rendu et recension critique d'ouvrage
DOI :
Titre :
Simultaneous genotyping of snails and infecting trematode parasites using high‐throughput amplicon sequencing
Auteur(s) :
Hammoud, Cyril [Auteur]
Royal Museum for Central Africa [Tervuren] [RMCA]
Universiteit Gent = Ghent University [UGENT]
Mulero, Stephen [Auteur]
Interactions Hôtes-Pathogènes-Environnements [IHPE]
Van Bocxlaer, Bert [Auteur]
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Universiteit Gent = Ghent University [UGENT]
Boissier, Jérôme [Auteur]
Interactions Hôtes-Pathogènes-Environnements [IHPE]
Verschuren, Dirk [Auteur]
Universiteit Gent = Ghent University [UGENT]
Albrecht, Christian [Auteur]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]
Huyse, Tine [Auteur]
Royal Museum for Central Africa [Tervuren] [RMCA]
Catholic University of Leuven = Katholieke Universiteit Leuven [KU Leuven]
Royal Museum for Central Africa [Tervuren] [RMCA]
Universiteit Gent = Ghent University [UGENT]
Mulero, Stephen [Auteur]
Interactions Hôtes-Pathogènes-Environnements [IHPE]
Van Bocxlaer, Bert [Auteur]
Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 [Evo-Eco-Paléo (EEP)]
Universiteit Gent = Ghent University [UGENT]
Boissier, Jérôme [Auteur]
Interactions Hôtes-Pathogènes-Environnements [IHPE]
Verschuren, Dirk [Auteur]
Universiteit Gent = Ghent University [UGENT]
Albrecht, Christian [Auteur]
Justus-Liebig-Universität Gießen = Justus Liebig University [JLU]
Huyse, Tine [Auteur]
Royal Museum for Central Africa [Tervuren] [RMCA]
Catholic University of Leuven = Katholieke Universiteit Leuven [KU Leuven]
Titre de la revue :
Molecular ecology resources
Pagination :
567-586
Éditeur :
Wiley/Blackwell
Date de publication :
2022-02
ISSN :
1755-098X
Mot(s)-clé(s) en anglais :
co-infections
community ecology
diagnostics
Gastropoda
high-throughput amplicon sequencing
schistosomiasis
community ecology
diagnostics
Gastropoda
high-throughput amplicon sequencing
schistosomiasis
Discipline(s) HAL :
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Parasitologie
Sciences du Vivant [q-bio]/Biologie animale/Médecine vétérinaire et santé animale
Sciences du Vivant [q-bio]/Ecologie, Environnement/Interactions entre organismes
Sciences du Vivant [q-bio]/Biologie animale/Médecine vétérinaire et santé animale
Sciences du Vivant [q-bio]/Ecologie, Environnement/Interactions entre organismes
Résumé en anglais : [en]
Several methodological issues currently hamper the study of entire trematode communities within populations of their intermediate snail hosts. Here we develop a new workflow using high-throughput amplicon sequencing to ...
Lire la suite >Several methodological issues currently hamper the study of entire trematode communities within populations of their intermediate snail hosts. Here we develop a new workflow using high-throughput amplicon sequencing to simultaneously genotype snail hosts and their infecting trematode parasites. We designed primers to amplify four snail and five trematode markers in a single multiplex PCR. While also applicable to other genera, we focused on medically and economically important snail genera within the superorder Hygrophila and targeted a broad taxonomic range of parasites within the class Trematoda. We tested the workflow using 417 Biomphalaria glabrata specimens experimentally infected with Schistosoma rodhaini, two strains of Schistosoma mansoni and combinations thereof. We evaluated the reliability of infection diagnostics, the robustness of the workflow, its specificity related to host and parasite identification, and the sensitivity to detect co-infections, immature infections and changes of parasite biomass during the infection process. Finally, we investigated its applicability in wild-caught snails of other genera naturally infected with a diverse range of trematodes. After stringent quality control the workflow allows the identification of snails to species level, and of trematodes to taxonomic levels ranging from family to strain. It is sensitive to detect immature infections and changes in parasite biomass described in previous experimental studies. Co-infections were successfully identified, opening the possibility to examine parasite-parasite interactions such as interspecific competition. Together, these results demonstrate that our workflow provides a powerful tool to analyse the processes shaping trematode communities within natural snail populations.Lire moins >
Lire la suite >Several methodological issues currently hamper the study of entire trematode communities within populations of their intermediate snail hosts. Here we develop a new workflow using high-throughput amplicon sequencing to simultaneously genotype snail hosts and their infecting trematode parasites. We designed primers to amplify four snail and five trematode markers in a single multiplex PCR. While also applicable to other genera, we focused on medically and economically important snail genera within the superorder Hygrophila and targeted a broad taxonomic range of parasites within the class Trematoda. We tested the workflow using 417 Biomphalaria glabrata specimens experimentally infected with Schistosoma rodhaini, two strains of Schistosoma mansoni and combinations thereof. We evaluated the reliability of infection diagnostics, the robustness of the workflow, its specificity related to host and parasite identification, and the sensitivity to detect co-infections, immature infections and changes of parasite biomass during the infection process. Finally, we investigated its applicability in wild-caught snails of other genera naturally infected with a diverse range of trematodes. After stringent quality control the workflow allows the identification of snails to species level, and of trematodes to taxonomic levels ranging from family to strain. It is sensitive to detect immature infections and changes in parasite biomass described in previous experimental studies. Co-infections were successfully identified, opening the possibility to examine parasite-parasite interactions such as interspecific competition. Together, these results demonstrate that our workflow provides a powerful tool to analyse the processes shaping trematode communities within natural snail populations.Lire moins >
Langue :
Anglais
Vulgarisation :
Non
Projet ANR :
Source :
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- Hammoud-MolEcolRes-Simultaneous-MANUSCRIT.pdf
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