Combined toxic effects and dna damage to ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Permalink :
Title :
Combined toxic effects and dna damage to two plant species exposed to binary metal mixtures (cd/pb)
Author(s) :
Lanier, C. [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Bernard, Fabien [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Dumez, Sylvain [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - EA 4483
Leclercq-Dransart, Julie [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Lemiere, Sébastien [Auteur]
Laboratoire Génie Civil et géo-Environnement (LGCgE) - ULR 4515
Vandenbulcke, Franck [Auteur]
Laboratoire Génie Civil et géo-Environnement (LGCgE) - ULR 4515
Nesslany, Fabrice [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Platel, Anne [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Devred, I. [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Hayet, A. [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Cuny, Damien [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - EA 4483
Deram, A. [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Bernard, Fabien [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Dumez, Sylvain [Auteur]
![refId](/themes/Mirage2//images/idref.png)
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - EA 4483
Leclercq-Dransart, Julie [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Lemiere, Sébastien [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Laboratoire Génie Civil et géo-Environnement (LGCgE) - ULR 4515
Vandenbulcke, Franck [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Laboratoire Génie Civil et géo-Environnement (LGCgE) - ULR 4515
Nesslany, Fabrice [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Platel, Anne [Auteur]
![refId](/themes/Mirage2//images/idref.png)
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Devred, I. [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Hayet, A. [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Cuny, Damien [Auteur]
![refId](/themes/Mirage2//images/idref.png)
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - EA 4483
Deram, A. [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Journal title :
Ecotoxicology and Environmental Safety
Abbreviated title :
Ecotoxicol. Environ. Saf.
Volume number :
167
Pages :
278-287
Publisher :
Elsevier
Publication date :
2018-10-18
ISSN :
1090-2414
Keyword(s) :
Antagonism
Genotoxicity
Metal mixture
Joint effects
Interactions
Genotoxicity
Metal mixture
Joint effects
Interactions
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Acute and long-term (3-, 10- and 56-day exposure) laboratory toxicity tests were carried out to assess the individual and combined toxic effects of cadmium (Cd) and lead (Pb) in Brassica oleracea and Trifolium repens. In ...
Show more >Acute and long-term (3-, 10- and 56-day exposure) laboratory toxicity tests were carried out to assess the individual and combined toxic effects of cadmium (Cd) and lead (Pb) in Brassica oleracea and Trifolium repens. In addition to morphological parameters, this work also used comet assay to address endpoints in relation to genotoxicity. Bioaccumulation was measured to demonstrate the influence of the mixture on the concentrations of each metal in the plant. The statistical method reported by Ince et al. (1999) was used to evaluate the types of interaction between Cd and Pb in each treatment and concerning their combined effect. This study concludes that the combined effects of binary metal combinations of Cd/Pb on morphological parameters are most often additive, sometimes antagonistic and more rarely synergistic, thus extending the findings of previous publications on this subject. DNA damage analysis revealed concentration- and time-dependent interactions. Synergistic effects of mixed metals (more breaks than individually applied metals) are observed in T. repens after a short exposure. Antagonistic effects are statistically significant after 10 days-exposure, suggesting competition between metals. At 56 days, the rate of DNA damage observed in plants exposed to the Cd/Pb mixture was similar to that measured in plants exposed to lead only and was significantly lower than the rate of DNA damage induced by Cd. This supports the idea that there may be competition between metals and also strengthens the hypothesis that long-term reparation mechanisms may be implemented. Cd/Pb co-exposure does not significantly influence the bioaccumulation of each metal. It is nevertheless important to note that a statistically significant 'interaction' is not necessarily biologically relevant and should therefore be considered with caution when assessing heavy metals combined effects.Show less >
Show more >Acute and long-term (3-, 10- and 56-day exposure) laboratory toxicity tests were carried out to assess the individual and combined toxic effects of cadmium (Cd) and lead (Pb) in Brassica oleracea and Trifolium repens. In addition to morphological parameters, this work also used comet assay to address endpoints in relation to genotoxicity. Bioaccumulation was measured to demonstrate the influence of the mixture on the concentrations of each metal in the plant. The statistical method reported by Ince et al. (1999) was used to evaluate the types of interaction between Cd and Pb in each treatment and concerning their combined effect. This study concludes that the combined effects of binary metal combinations of Cd/Pb on morphological parameters are most often additive, sometimes antagonistic and more rarely synergistic, thus extending the findings of previous publications on this subject. DNA damage analysis revealed concentration- and time-dependent interactions. Synergistic effects of mixed metals (more breaks than individually applied metals) are observed in T. repens after a short exposure. Antagonistic effects are statistically significant after 10 days-exposure, suggesting competition between metals. At 56 days, the rate of DNA damage observed in plants exposed to the Cd/Pb mixture was similar to that measured in plants exposed to lead only and was significantly lower than the rate of DNA damage induced by Cd. This supports the idea that there may be competition between metals and also strengthens the hypothesis that long-term reparation mechanisms may be implemented. Cd/Pb co-exposure does not significantly influence the bioaccumulation of each metal. It is nevertheless important to note that a statistically significant 'interaction' is not necessarily biologically relevant and should therefore be considered with caution when assessing heavy metals combined effects.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
CHU Lille
IMT Lille Douai
Institut Catholique Lille
Institut Pasteur de Lille
Univ. Artois
Université de Lille
IMT Lille Douai
Institut Catholique Lille
Institut Pasteur de Lille
Univ. Artois
Université de Lille
Collections :
Submission date :
2022-02-02T10:23:45Z
2024-03-13T08:43:49Z
2024-03-13T08:43:49Z