Evaluation of amazonian fungi biomass as ...
Document type :
Article dans une revue scientifique: Article original
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Title :
Evaluation of amazonian fungi biomass as a source of lipases for biocatalysis
Author(s) :
Romano, Israel P. [Auteur]
Dos Santos, Vanderlei S. [Auteur]
De Lima Paes Louzada, Ana Carolina [Auteur]
Pereira, Junior Raimundo C. [Auteur]
Do Carmo, Edson J. [Auteur]
Da Mota, Adolfo Jose [Auteur]
Barroso, Hileia Dos S. [Auteur]
Itabaiana, Ivaldo [Auteur]
Pereira, Jose Odair [Auteur]
Astolfi, Filho Spartaco [Auteur]
Zanotto, Sandra Patricia [Auteur]
Dos Santos, Vanderlei S. [Auteur]
De Lima Paes Louzada, Ana Carolina [Auteur]
Pereira, Junior Raimundo C. [Auteur]
Do Carmo, Edson J. [Auteur]
Da Mota, Adolfo Jose [Auteur]
Barroso, Hileia Dos S. [Auteur]
Itabaiana, Ivaldo [Auteur]
Pereira, Jose Odair [Auteur]
Astolfi, Filho Spartaco [Auteur]
Zanotto, Sandra Patricia [Auteur]
Journal title :
Quimica nova
Abbreviated title :
Quim. Nova
Volume number :
43
Pages :
146-154
Publication date :
2020-02-03
ISSN :
0100-4042
English keyword(s) :
transesterification
amazonian fungi
enantioselectivity
biocatalysis
lipase
biomass
amazonian fungi
enantioselectivity
biocatalysis
lipase
biomass
HAL domain(s) :
Chimie/Catalyse
English abstract : [en]
We evaluated the biomass of twenty Amazonian fungal isolates as a potential source of mycelium-bound lipases with hydrolytic, synthetic or enantioselective activity for biocatalysis application. We compared the hydrolytic ...
Show more >We evaluated the biomass of twenty Amazonian fungal isolates as a potential source of mycelium-bound lipases with hydrolytic, synthetic or enantioselective activity for biocatalysis application. We compared the hydrolytic activity of three biomass treatments (delipidated, non-delipidated and cultivated in medium without inducer). Delipidated biomass showed better results in the hydrolysis of p-nitrophenyl palmitate compared to the other two treatments for fifteen isolates. Delipidated biomass of six Aspergillus strains and UEA_115 strain showed a high synthesis activity of ethyl palmitate by transesterification in organic medium. Results were confirmed by spectrophotometry (410 nm) and gas chromatography. In this reaction, the isolate DPUA_1539 A. flavo-furcatis reached a maximum value of 668.5 ± 23.5 mU g-1. Enantioselective activity assays indicated that biomass-bound lipases from UEA_115 isolate (E = 3.58; ees = 7 ± 0) and in particular from the DPUA_1539 A. flavo-furcatis isolate (E = 24.15; ees = 91 ± 1) have the ability to discriminate enantiomers of the drug ibuprofen by ester synthesis, preferably with (R)-enantiomer. These results encourage further investigations of these fungi as potential lipase suppliers for biocatalytic processes such as biodiesel production and enantiopure drugs.Show less >
Show more >We evaluated the biomass of twenty Amazonian fungal isolates as a potential source of mycelium-bound lipases with hydrolytic, synthetic or enantioselective activity for biocatalysis application. We compared the hydrolytic activity of three biomass treatments (delipidated, non-delipidated and cultivated in medium without inducer). Delipidated biomass showed better results in the hydrolysis of p-nitrophenyl palmitate compared to the other two treatments for fifteen isolates. Delipidated biomass of six Aspergillus strains and UEA_115 strain showed a high synthesis activity of ethyl palmitate by transesterification in organic medium. Results were confirmed by spectrophotometry (410 nm) and gas chromatography. In this reaction, the isolate DPUA_1539 A. flavo-furcatis reached a maximum value of 668.5 ± 23.5 mU g-1. Enantioselective activity assays indicated that biomass-bound lipases from UEA_115 isolate (E = 3.58; ees = 7 ± 0) and in particular from the DPUA_1539 A. flavo-furcatis isolate (E = 24.15; ees = 91 ± 1) have the ability to discriminate enantiomers of the drug ibuprofen by ester synthesis, preferably with (R)-enantiomer. These results encourage further investigations of these fungi as potential lipase suppliers for biocatalytic processes such as biodiesel production and enantiopure drugs.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
CNRS
Centrale Lille
ENSCL
Univ. Artois
Université de Lille
Centrale Lille
ENSCL
Univ. Artois
Université de Lille
Collections :
Submission date :
2022-03-02T07:14:36Z