Localized intrathecal delivery of mesenchymal ...
Document type :
Article dans une revue scientifique: Article original
DOI :
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Title :
Localized intrathecal delivery of mesenchymal stromal cells conditioned medium improves functional recovery in a rat model of spinal cord injury
Author(s) :
Cizkova, Dasa [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Cubinkova, Veronika [Auteur]
Slovak Academy of Sciences [SAS]
Smolek, Tomas [Auteur]
Slovak Academy of Sciences [SAS]
Murgoci, Adriana-Natalia [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Danko, Jan [Auteur]
University of Veterinary Medicine and Pharmacy [Košice, Slovakia]
Vdoviakova, Katarina [Auteur]
University of Veterinary Medicine and Pharmacy [Košice, Slovakia]
Humenik, Filip [Auteur]
University of Veterinary Medicine and Pharmacy [Košice, Slovakia]
Cizek, Milan [Auteur]
University of Veterinary Medicine and Pharmacy [Košice, Slovakia]
Quanico, Jusal [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
FOURNIER, Isabelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Salzet, Michel [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
![refId](/themes/Mirage2//images/idref.png)
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Cubinkova, Veronika [Auteur]
Slovak Academy of Sciences [SAS]
Smolek, Tomas [Auteur]
Slovak Academy of Sciences [SAS]
Murgoci, Adriana-Natalia [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Danko, Jan [Auteur]
University of Veterinary Medicine and Pharmacy [Košice, Slovakia]
Vdoviakova, Katarina [Auteur]
University of Veterinary Medicine and Pharmacy [Košice, Slovakia]
Humenik, Filip [Auteur]
University of Veterinary Medicine and Pharmacy [Košice, Slovakia]
Cizek, Milan [Auteur]
University of Veterinary Medicine and Pharmacy [Košice, Slovakia]
Quanico, Jusal [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
FOURNIER, Isabelle [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Salzet, Michel [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Journal title :
International Journal of Molecular Sciences
Abbreviated title :
Int J Mol Sci
Volume number :
19
Pages :
870
Publication date :
2018-03-15
ISSN :
1661-6596
Keyword(s) :
spinal cord injury
stem cells-derived conditioned media
regeneration
inflammation
stem cells-derived conditioned media
regeneration
inflammation
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
It was recently shown that the conditioned medium (CM) of mesenchymal stem cells can enhance viability of neural and glial cell populations. In the present study, we have investigated a cell-free approach via CM from rat ...
Show more >It was recently shown that the conditioned medium (CM) of mesenchymal stem cells can enhance viability of neural and glial cell populations. In the present study, we have investigated a cell-free approach via CM from rat bone marrow stromal cells (MScCM) applied intrathecally (IT) for spinal cord injury (SCI) recovery in adult rats. Functional in vitro test on dorsal root ganglion (DRG) primary cultures confirmed biological properties of collected MScCM for production of neurosphere-like structures and axon outgrowth. Afterwards, rats underwent SCI and were treated with IT delivery of MScCM or vehicle at postsurgical Days 1, 5, 9, and 13, and left to survive 10 weeks. Rats that received MScCM showed significantly higher motor function recovery, increase in spared spinal cord tissue, enhanced GAP-43 expression and attenuated inflammation in comparison with vehicle-treated rats. Spared tissue around the lesion site was infiltrated with GAP-43-labeled axons at four weeks that gradually decreased at 10 weeks. Finally, a cytokine array performed on spinal cord extracts after MScCM treatment revealed decreased levels of IL-2, IL-6 and TNFalpha when compared to vehicle group. In conclusion, our results suggest that molecular cocktail found in MScCM is favorable for final neuroregeneration after SCI.Show less >
Show more >It was recently shown that the conditioned medium (CM) of mesenchymal stem cells can enhance viability of neural and glial cell populations. In the present study, we have investigated a cell-free approach via CM from rat bone marrow stromal cells (MScCM) applied intrathecally (IT) for spinal cord injury (SCI) recovery in adult rats. Functional in vitro test on dorsal root ganglion (DRG) primary cultures confirmed biological properties of collected MScCM for production of neurosphere-like structures and axon outgrowth. Afterwards, rats underwent SCI and were treated with IT delivery of MScCM or vehicle at postsurgical Days 1, 5, 9, and 13, and left to survive 10 weeks. Rats that received MScCM showed significantly higher motor function recovery, increase in spared spinal cord tissue, enhanced GAP-43 expression and attenuated inflammation in comparison with vehicle-treated rats. Spared tissue around the lesion site was infiltrated with GAP-43-labeled axons at four weeks that gradually decreased at 10 weeks. Finally, a cytokine array performed on spinal cord extracts after MScCM treatment revealed decreased levels of IL-2, IL-6 and TNFalpha when compared to vehicle group. In conclusion, our results suggest that molecular cocktail found in MScCM is favorable for final neuroregeneration after SCI.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
INSERM
Université de Lille
Université de Lille
Submission date :
2022-06-15T13:58:28Z
2023-01-25T09:49:33Z
2023-01-25T09:49:33Z
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