Title :

Simple gene signature to assess murine fibroblast polarization

Author(s) :

Ledoult, Emmanuel [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Centre National de Référence des Maladies Auto-Immunes Systémiques Rares du Nord et Nord-Ouest de France [CeRAINO]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Jendoubi, Manel [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Collet, Aurore [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Centre National de Référence des Maladies Auto-Immunes Systémiques Rares du Nord et Nord-Ouest de France [CeRAINO]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Guerrier, Thomas [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Largy, Alexis [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Speca, Silvia [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Vivier, Solange [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Bray, Fabrice [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Figeac, Martin [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Hachulla, Eric [Auteur]
Centre National de Référence des Maladies Auto-Immunes Systémiques Rares du Nord et Nord-Ouest de France [CeRAINO]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Labalette, Myriam [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Leprêtre, Frédéric [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Sebda, Shéhérazade [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Sanges, Sébastien [Auteur]
Centre National de Référence des Maladies Auto-Immunes Systémiques Rares du Nord et Nord-Ouest de France [CeRAINO]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Rolando, Christian [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Sobanski, Vincent [Auteur]
Centre National de Référence des Maladies Auto-Immunes Systémiques Rares du Nord et Nord-Ouest de France [CeRAINO]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Institut universitaire de France [IUF]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Dubucquoi, Sylvain [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Launay, David [Auteur]
Centre National de Référence des Maladies Auto-Immunes Systémiques Rares du Nord et Nord-Ouest de France [CeRAINO]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]

Journal title :

Scientific Reports

Pages :

11748

Publisher :

Nature Publishing Group

Publication date :

2022-12

ISSN :

2045-2322

English keyword(s) :

fibroblast
polarization
Omics

HAL domain(s) :

Sciences du Vivant [q-bio]/Biologie cellulaire/Interactions cellulaires [q-bio.CB]

English abstract : [en]

We provide an original multi-stage approach identifying a gene signature to assess murine fibroblast polarization. Prototypic polarizations (inflammatory/fibrotic) were induced by seeded mouse embryonic fibroblasts (MEFs) ...
Show more >We provide an original multi-stage approach identifying a gene signature to assess murine fibroblast polarization. Prototypic polarizations (inflammatory/fibrotic) were induced by seeded mouse embryonic fibroblasts (MEFs) with TNFα or TGFß1, respectively. The transcriptomic and proteomic profiles were obtained by RNA microarray and LC-MS/MS. Gene Ontology and pathways analysis were performed among the differentially expressed genes (DEGs) and proteins (DEPs). Balb/c mice underwent daily intradermal injections of HOCl (or PBS) as an experimental murine model of inflammation-mediated fibrosis in a time-dependent manner. As results, 1456 and 2215 DEGs, and 289 and 233 DEPs were respectively found in MEFs in response to TNFα or TGFß1, respectively . Among the most significant pathways, we combined 26 representative genes to encompass the proinflammatory and profibrotic polarizations of fibroblasts. Based on principal component analysis, this signature deciphered baseline state, proinflammatory polarization, and profibrotic polarization as accurately as RNA microarray and LC-MS/MS did. Then, we assessed the gene signature on dermal fibroblasts isolated from the experimental murine model. We observed a proinflammatory polarization at day 7, and a mixture of a proinflammatory and profibrotic polarizations at day 42 in line with histological findings. Our approach provides a small-size and convenient gene signature to assess murine fibroblast polarization.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Miniaturisation pour la Synthèse, l'Analyse et la Protéomique (MSAP) - USR 3290

Source :

Harvested from HAL