Passages in culture and stimulation ...
Document type :
Compte-rendu et recension critique d'ouvrage
DOI :
PMID :
Title :
Passages in culture and stimulation conditions influence protein expression of primary fibroblasts
Author(s) :
Vivier, Solange [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Chepy, Aurélien [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Centre National de Référence des Maladies Auto-Immunes Systémiques Rares du Nord et Nord-Ouest de France [CeRAINO]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Bray, Fabrice [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Guerrier, Thomas [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Institut d'Immunologie [CHRU Lille]
Speca, Silvia [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Flament, Stéphanie [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Jendoubi, Manel [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Balden, Maïté [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Institut d'Immunologie [CHRU Lille]
Rolando, Christian [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Hachulla, Eric [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Centre National de Référence des Maladies Auto-Immunes Systémiques Rares du Nord et Nord-Ouest de France [CeRAINO]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Launay, David [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Dubucquoi, Sylvain [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Institut d'Immunologie [CHRU Lille]
Sobanski, Vincent [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Centre National de Référence des Maladies Auto-Immunes Systémiques Rares du Nord et Nord-Ouest de France [CeRAINO]
Institut universitaire de France [IUF]
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Chepy, Aurélien [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Centre National de Référence des Maladies Auto-Immunes Systémiques Rares du Nord et Nord-Ouest de France [CeRAINO]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Bray, Fabrice [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Guerrier, Thomas [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Institut d'Immunologie [CHRU Lille]
Speca, Silvia [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Flament, Stéphanie [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Jendoubi, Manel [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Balden, Maïté [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Institut d'Immunologie [CHRU Lille]
Rolando, Christian [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Hachulla, Eric [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Centre National de Référence des Maladies Auto-Immunes Systémiques Rares du Nord et Nord-Ouest de France [CeRAINO]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Launay, David [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Dubucquoi, Sylvain [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Institut d'Immunologie [CHRU Lille]
Sobanski, Vincent [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Institute for Translational Research in Inflammation - U 1286 [INFINITE (Ex-Liric)]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Centre National de Référence des Maladies Auto-Immunes Systémiques Rares du Nord et Nord-Ouest de France [CeRAINO]
Institut universitaire de France [IUF]
Journal title :
Proteomics
Pages :
2100116
Publisher :
Wiley-VCH Verlag
Publication date :
2022-02
ISSN :
1615-9853
English keyword(s) :
fibroblasts
systemic sclerosis
Proteomics
systemic sclerosis
Proteomics
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Fibroblasts (Fb) are key effector cells in systemic sclerosis (SSc). Fb stimulation with transforming growth factor beta 1 (TGF-β1) is considered as a positive control in studies assessing fibrogenesis. The lack of ...
Show more >Fibroblasts (Fb) are key effector cells in systemic sclerosis (SSc). Fb stimulation with transforming growth factor beta 1 (TGF-β1) is considered as a positive control in studies assessing fibrogenesis. The lack of standardization of TGF-β1 stimulation might be responsible for discrepancies in experiments performed in different conditions. Using quantitative proteomics analysis, we evaluated the impact of changes in experimental conditions on proteomic profiles of primary Fb. Principal component analysis (PCA) identified several groups of differentially expressed proteins influenced by cell passage, culture medium, and both concentration and duration of exposure to TGF-β1 stimulation. Bioinformatics analysis revealed that late passages expressed proteins involved in senescence. TGF-β1 concentration and time of stimulation were correlated with the expression of proteins involved in the fibrogenesis and inflammatory processes. These data underline the need for standardization of culture conditions to allow inter-data comparisons in future in vitro studies, especially when using “omics” approaches.Show less >
Show more >Fibroblasts (Fb) are key effector cells in systemic sclerosis (SSc). Fb stimulation with transforming growth factor beta 1 (TGF-β1) is considered as a positive control in studies assessing fibrogenesis. The lack of standardization of TGF-β1 stimulation might be responsible for discrepancies in experiments performed in different conditions. Using quantitative proteomics analysis, we evaluated the impact of changes in experimental conditions on proteomic profiles of primary Fb. Principal component analysis (PCA) identified several groups of differentially expressed proteins influenced by cell passage, culture medium, and both concentration and duration of exposure to TGF-β1 stimulation. Bioinformatics analysis revealed that late passages expressed proteins involved in senescence. TGF-β1 concentration and time of stimulation were correlated with the expression of proteins involved in the fibrogenesis and inflammatory processes. These data underline the need for standardization of culture conditions to allow inter-data comparisons in future in vitro studies, especially when using “omics” approaches.Show less >
Language :
Anglais
Popular science :
Non
Source :