Generation of β-like cell subtypes from ...
Type de document :
Compte-rendu et recension critique d'ouvrage
DOI :
Titre :
Generation of β-like cell subtypes from differentiated human induced pluripotent stem cells in 3D spheroids
Auteur(s) :
Morisseau, Lisa [Auteur]
Biomécanique et Bioingénierie [BMBI]
Tokito, Fumiya [Auteur]
Graduate School of Engineering [The Univ of Tokyo] [UTokyo]
Poulain, Stéphane [Auteur]
Institute of Industrial Science [IIS]
Plaisance, Valérie [Auteur]
NanoBioInterfaces - IEMN [NBI - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Pawlowski, Valerie [Auteur]
NanoBioInterfaces - IEMN [NBI - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Kim, Soo Hyeon [Auteur]
Institute of Industrial Science [IIS]
Legallais, Cecile [Auteur]
Biomécanique et Bioingénierie [BMBI]
Jellali, Rachid [Auteur]
Biomécanique et Bioingénierie [BMBI]
Sakai, Yasuyuki [Auteur]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
Abderrahmani, Amar [Auteur correspondant]
NanoBioInterfaces - IEMN [NBI - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Leclerc, Eric [Auteur correspondant]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
Biomécanique et Bioingénierie [BMBI]
Biomécanique et Bioingénierie [BMBI]
Tokito, Fumiya [Auteur]
Graduate School of Engineering [The Univ of Tokyo] [UTokyo]
Poulain, Stéphane [Auteur]
Institute of Industrial Science [IIS]
Plaisance, Valérie [Auteur]
NanoBioInterfaces - IEMN [NBI - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Pawlowski, Valerie [Auteur]
NanoBioInterfaces - IEMN [NBI - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Kim, Soo Hyeon [Auteur]
Institute of Industrial Science [IIS]
Legallais, Cecile [Auteur]
Biomécanique et Bioingénierie [BMBI]
Jellali, Rachid [Auteur]
Biomécanique et Bioingénierie [BMBI]
Sakai, Yasuyuki [Auteur]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
Abderrahmani, Amar [Auteur correspondant]
NanoBioInterfaces - IEMN [NBI - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Leclerc, Eric [Auteur correspondant]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
Biomécanique et Bioingénierie [BMBI]
Titre de la revue :
Molecular Omics
Pagination :
810-822
Éditeur :
Royal Society of Chemistry
Date de publication :
2023-12-01
Discipline(s) HAL :
Chimie
Sciences du Vivant [q-bio]/Biologie cellulaire
Sciences de l'ingénieur [physics]
Sciences du Vivant [q-bio]/Biologie cellulaire
Sciences de l'ingénieur [physics]
Résumé en anglais : [en]
Since the identification of four different pancreatic β-cell subtypes and bi-hormomal cells playing a role in the diabetes pathogenesis, the search for in vitro models that mimics such cells heterogeneity became a key ...
Lire la suite >Since the identification of four different pancreatic β-cell subtypes and bi-hormomal cells playing a role in the diabetes pathogenesis, the search for in vitro models that mimics such cells heterogeneity became a key priority in experimental and clinical diabetology. We investigated the potential of human induced pluripotent stem cells to lead to the development of the different β-cells subtypes in honeycomb microwell-based 3D spheroids. The glucose-stimulated insulin secretion confirmed the spheroids functionality. Then, we performed a single cell RNA sequencing of the spheroids. Using a knowledge-based analysis with a stringency on the pancreatic markers, we extracted the β-cells INS+/UCN3+ subtype (11%; β1-like cells), the INS+/ST8SIA1+/CD9− subtype (3%, β3-like cells) and INS+/CD9+/ST8SIA1-subtype (1%; β2-like cells) consistently with literature findings. We did not detect the INS+/ST8SIA1+/CD9+ cells (β4-like cells). Then, we also identified four bi-hormonal cells subpopulations including δ-like cells (INS+/SST+, 6%), γ-like cells (INS+/PPY+, 3%), α-like-cells (INS+/GCG+, 6%) and ε-like-cells (INS+/GHRL+, 2%). Using data-driven clustering, we extracted four progenitors’ subpopulations (with the lower level of INS gene) that included one population highly expressing inhibin genes (INHBA+/INHBB+), one population highly expressing KCNJ3+/TPH1+, one population expressing hepatocyte-like lineage markers (HNF1A+/AFP+), and one population expressing stem-like cell pancreatic progenitor markers (SOX2+/NEUROG3+). Furthermore, among the cycling population we found a large number of REST+ cells and CD9+ cells (CD9+/SPARC+/REST+). Our data confirm that our differentiation leads to large β-cell heterogeneity, which can be used for investigating β-cells plasticity under physiological and pathophysiological conditions.Lire moins >
Lire la suite >Since the identification of four different pancreatic β-cell subtypes and bi-hormomal cells playing a role in the diabetes pathogenesis, the search for in vitro models that mimics such cells heterogeneity became a key priority in experimental and clinical diabetology. We investigated the potential of human induced pluripotent stem cells to lead to the development of the different β-cells subtypes in honeycomb microwell-based 3D spheroids. The glucose-stimulated insulin secretion confirmed the spheroids functionality. Then, we performed a single cell RNA sequencing of the spheroids. Using a knowledge-based analysis with a stringency on the pancreatic markers, we extracted the β-cells INS+/UCN3+ subtype (11%; β1-like cells), the INS+/ST8SIA1+/CD9− subtype (3%, β3-like cells) and INS+/CD9+/ST8SIA1-subtype (1%; β2-like cells) consistently with literature findings. We did not detect the INS+/ST8SIA1+/CD9+ cells (β4-like cells). Then, we also identified four bi-hormonal cells subpopulations including δ-like cells (INS+/SST+, 6%), γ-like cells (INS+/PPY+, 3%), α-like-cells (INS+/GCG+, 6%) and ε-like-cells (INS+/GHRL+, 2%). Using data-driven clustering, we extracted four progenitors’ subpopulations (with the lower level of INS gene) that included one population highly expressing inhibin genes (INHBA+/INHBB+), one population highly expressing KCNJ3+/TPH1+, one population expressing hepatocyte-like lineage markers (HNF1A+/AFP+), and one population expressing stem-like cell pancreatic progenitor markers (SOX2+/NEUROG3+). Furthermore, among the cycling population we found a large number of REST+ cells and CD9+ cells (CD9+/SPARC+/REST+). Our data confirm that our differentiation leads to large β-cell heterogeneity, which can be used for investigating β-cells plasticity under physiological and pathophysiological conditions.Lire moins >
Langue :
Anglais
Vulgarisation :
Non
Commentaire :
Sequencing data supporting the findings presented in this study were deposited at Zenodo (https://zenodo.org) with the following Digital Object Identifier: 10.5281/zenodo.7960673. All supplementary table data files and supplementary figures prepared for this study are available in the ESI supplementary files provided to the journal.
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