Title :

Changes in CRH and ACTH synthesis during experimental and human septic shock.

Author(s) :

Polito, Andrea [Auteur]
Sonneville, Romain [Auteur]
Guidoux, Céline [Auteur]
Barrett, Lucinda [Auteur]
Viltart, Odile [Auteur]
Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U1172 Inserm - U837 [JPArc]
Mattot, Virginie [Auteur]
Siami, Shidasp [Auteur]
Lorin de la Grandmaison, Geoffroy [Auteur]
Chrétien, Fabrice [Auteur]
Singer, Mervyn [Auteur]
Gray, Françoise [Auteur]
Annane, Djillali [Auteur]
Brouland, Jean-Philippe [Auteur]
Sharshar, Tarek [Auteur]

Journal title :

PLOS ONE

Abbreviated title :

PLoS One

Volume number :

6

Pages :

e25905

Publication date :

2011-11-03

ISSN :

1932-6203

English keyword(s) :

Adrenocorticotropic Hormone
Animals
Arginine Vasopressin
Corticotropin-Releasing Hormone
Disease Models, Animal
Humans
Immunohistochemistry
In Situ Hybridization
Nitric Oxide Synthase Type II
RNA, Messenger
Rats
Receptors, Vasopressin
Shock, Septic

HAL domain(s) :

Sciences cognitives

English abstract : [en]

The mechanisms of septic shock-associated adrenal insufficiency remain unclear. This study aimed at investigating the synthesis of corticotropin-releasing hormone (CRH) and vasopressin (AVP) by parvocellular neurons and ...
Show more >The mechanisms of septic shock-associated adrenal insufficiency remain unclear. This study aimed at investigating the synthesis of corticotropin-releasing hormone (CRH) and vasopressin (AVP) by parvocellular neurons and the antehypophyseal expression of ACTH in human septic shock and in an experimental model of sepsis. To test the hypothesis that ACTH secretion is decreased secondarily to alteration of CRH or AVP synthesis, we undertook a neuropathological study of the antehypophyseal system in patients who had died from septic shock and rats with experimental faecal peritonitis. Brains obtained in 9 septic shock patients were compared to 10 nonseptic patients (controls). Parvocellular expression of AVP and CRH mRNA were evaluated by in situ hybridization. Antehypophyseal expression of ACTH, vasopressin V1b and CRH R1 receptors and parvocellular expression of iNOS in the PVN were evaluated by immunohistochemistry. The same experiments were carried out in a fecal peritonitis-induced model of sepsis. Data from septic rats with (n = 6) or without (n = 10) early death were compared to sham-operated (n = 8) animals. In patients and rats, septic shock was associated with a decreased expression of ACTH, unchanged expression of V1B receptor, CRHR1 and AVP mRNA, and increased expression of parvocellular iNOS compared to controls. Septic shock was also characterized by an increased expression of CRH mRNA in rats but not in patients, who notably had a greater duration of septic shock. The present study suggests that in humans and in rats, septic shock is associated with decreased ACTH synthesis that is not compensated by its two natural secretagogues, AVP and CRH. One underlying mechanism might be increased expression of iNOS in hypothalamic parvocellular neurons.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS
CHU Lille

Collections :

• Sciences Cognitives et Sciences Affectives (SCALab) - UMR 9193

Submission date :

2024-01-12T14:33:33Z