Variation of ewe olfactory secretome during a ram effect

Cann, Paul; Le Danvic, Chrystelle; Porte, Chantal; Chesneau, Didier; Keller, Matthieu; Nagnan-Le Meillour, Patricia

Document type :

Article dans une revue scientifique

DOI :

10.3389/fvets.2022.1033412

Permalink :

http://hdl.handle.net/20.500.12210/106563

Title :

Variation of ewe olfactory secretome during a ram effect

Author(s) :

Cann, Paul [Auteur]
Le Danvic, Chrystelle [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Porte, Chantal [Auteur]
Chesneau, Didier [Auteur]
Keller, Matthieu [Auteur]
Nagnan-Le Meillour, Patricia [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Journal title :

Frontiers in Veterinary Science

Abbreviated title :

Front. Vet. Sci.

Publisher :

Frontiers Media SA

Publication date :

2023-01-09

ISSN :

2297-1769

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

IntroductionUnder temperate latitudes, reproduction in Ovis aries displays a marked seasonality, governed by the photoperiod. In natural conditions, the transition between sexual rest and sexual activity in both sexes is ...
Show more >IntroductionUnder temperate latitudes, reproduction in Ovis aries displays a marked seasonality, governed by the photoperiod. In natural conditions, the transition between sexual rest and sexual activity in both sexes is induced by the decrease of day light. Meanwhile, specific odors emitted by a sexually active male are able to reactivate the gonadotropic axis of anovulatory ewes. This physiological effect is called “male effect”, precisely ram effect in the ovine species. We have previously shown that the secreted proteins, namely Olfactory Binding Proteins (OBP), contained in the nasal mucus constitute the olfactory secretome (OS), the composition of which is determined by the status of oestrus cycle of females and differs between sexual rest and sexual activity periods. The objective of this study was to test the hypothesis that exposure to sexually active male can also modify the composition of ewes olfactory secretome during a male effect, as well as hormones produced by the reactivation of the oestrus cycle in sexual activity period under natural conditions.MethodsWe have set up a new non-invasive protocol of nasal mucus sampling and collected it from 12 ewes at different times during a ram effect. We analyzed the composition of their olfactory secretome by proteomics, mainly SDS-PAGE and MALDI-TOF mass spectrometry. As post-translational modifications of OBPs were a hallmark of ewes' sexual activity period, we were looking for glycosylation by western-blot and mass spectrometry.ResultsThe efficiency of male effect was low in stimulated ewes as only 3 females displayed elevated progesterone levels in their blood. Besides, half of control ewes (non-stimulated ones) were cycled. We noticed a common OS profile in ewes in anoestrus, versus OS of cycled ones. A very clear and important result was the apparition of O-GlcNAcylation, previously detected only in sexual activity, after only 30 min of male introduction into the flock.DiscussionThis exploratory study paves the way for further experiments with larger flock to confirm and reinforce these results, and for eventually exploiting the nasal mucus as an indicator of females' receptivity to male odors.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Glycobiologie de l’olfaction

Submission date :

2024-02-07T15:25:44Z

Files

P23.47 fvets-09-1033412.pdf
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Version	Link	Modification date
3	20.500.12210/106563	2024-02-23T08:53:00Z
2	20.500.12210/106563.2	2024-02-23T08:47:49Z
1	20.500.12210/106563.1*	2024-02-07T15:25:44Z

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Variation of ewe olfactory secretome during ...

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