Title :

Palmitate increases Nur77 expression by modulating ZBP89 and Sp1 binding to the Nur77 proximal promoter in pancreatic β‐cells

Author(s) :

Mazuy, Claire [Auteur]
Ploton, Maheul [Auteur]
Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U 1011 [RNMCD]
Eeckhoute, Jérôme [Auteur]
Berrabah, Wahiba [Auteur]
Staels, Bart [Auteur]
Lefebvre, Philippe [Auteur]
Helleboid-Chapman, Audrey [Auteur]

Journal title :

Febs Letters

Pages :

3883-3890

Publisher :

Wiley

Publication date :

2013-10-25

ISSN :

0014-5793

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Nur77 is a stress sensor in pancreatic β‐cells, which negatively regulates glucose‐stimulated insulin secretion. We recently showed that a lipotoxic shock caused by exposure of β‐cells to the saturated fatty acid palmitate ...
Show more >Nur77 is a stress sensor in pancreatic β‐cells, which negatively regulates glucose‐stimulated insulin secretion. We recently showed that a lipotoxic shock caused by exposure of β‐cells to the saturated fatty acid palmitate strongly increases Nur77 expression. Here, using dual luciferase reporter assays and Nur77 promoter deletion constructs, we identified a regulatory cassette between −1534 and −1512 bp upstream from the translational start site mediating Nur77 promoter activation in response to palmitate exposure. Chromatin immunoprecipitation, transient transfection and siRNA‐mediated knockdown assays revealed that palmitate induced Nur77 promoter activation involves Sp1 recruitment and ZBP89 release from the gene promoter.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Récepteurs nucléaires, Maladies Cardiovasculaires et Diabète (RNMCD) - U1011

Source :

Harvested from HAL