Title :

Pairing cells of different sizes in a microfluidic device for immunological synapse monitoring

Author(s) :

Shaik, Faruk Azam [Auteur]
Université de Lille
Centre National de la Recherche Scientifique [CNRS]
Lewuillon, Clara [Auteur]
Université de Lille
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Guillemette, Aurélie [Auteur]
Université de Lille
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Ahmadian, Bahram [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Bio-Micro-Electro-Mechanical Systems - IEMN [BIOMEMS - IEMN]
JUNIA [JUNIA]
Brinster, Carine [Auteur]
Université de Lille
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Quesnel, Bruno [Auteur]
Université de Lille
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Collard, Dominique [Auteur]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
Touil, Yasmine [Auteur]
Université de Lille
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Lemonnier, Loïc [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Tarhan, Mehmet-Cagatay [Auteur]
Bio-Micro-Electro-Mechanical Systems - IEMN [BIOMEMS - IEMN]
JUNIA [JUNIA]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]

Journal title :

Lab on a Chip

Pages :

908-920

Publisher :

Royal Society of Chemistry

Publication date :

2022

ISSN :

1473-0197

HAL domain(s) :

Sciences du Vivant [q-bio]/Immunologie
Sciences du Vivant [q-bio]/Biologie cellulaire
Sciences de l'ingénieur [physics]/Micro et nanotechnologies/Microélectronique

English abstract : [en]

Analyzing cell–cell interaction is essential to investigate how immune cells function. Elegant designs have been demonstrated to study lymphocytes and their interaction partners. However, these devices have been targeting ...
Show more >Analyzing cell–cell interaction is essential to investigate how immune cells function. Elegant designs have been demonstrated to study lymphocytes and their interaction partners. However, these devices have been targeting cells of similar dimensions. T lymphocytes are smaller, more deformable, and more sensitive to pressure than many cells. This work aims to fill the gap of a method for pairing cells with different dimensions. The developed method uses hydrodynamic flow focusing in the z-direction for on-site modulation of effective channel height to capture smaller cells as single cells. Due to immune cells' sensitivity to pressure, the proposed method provides a stable system without any change in flow conditions at the analysis area throughout experiments. Paired live cells have their activities analyzed with calcium imaging at the immunological synapse formed under a controlled environment. The method is demonstrated with primary human T lymphocytes, acute myeloid leukemia (AML) cell lines, and primary AML blasts.Show less >

Language :

Anglais

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL

Submission date :

2024-02-17T03:50:28Z