Scurvy presenting as pseudo-scleroderma of the leg
Document type :
Compte-rendu et recension critique d'ouvrage
Title :
Scurvy presenting as pseudo-scleroderma of the leg
Author(s) :
Ahangari, Dario [Auteur]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Gillard, Manon [Auteur]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Certad, Gabriela [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Bataille, Mathieu [Auteur]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Lasek, Audrey [Auteur]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Modiano, Phillippe [Auteur]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Gillard, Manon [Auteur]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Certad, Gabriela [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Bataille, Mathieu [Auteur]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Lasek, Audrey [Auteur]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Modiano, Phillippe [Auteur]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Journal title :
JAAD Case reports
Pages :
831-833
Publisher :
Elsevier
Publication date :
2019-09
ISSN :
2352-5126
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Ex vivo explant culture models offer unique properties to study complex mechanisms underlying tissue growth, renewal, and disease. A major weakness is the short viability depending on the biopsy origin and preparation ...
Show more >Ex vivo explant culture models offer unique properties to study complex mechanisms underlying tissue growth, renewal, and disease. A major weakness is the short viability depending on the biopsy origin and preparation protocol. We describe an interphase microfluidic culture system to cultivate full thickness murine colon explants which keeps morphological structures of the tissue up to 192 h. The system was composed of a central well on top of a porous membrane supported by a microchannel structure. The microfluidic perfusion allowed bathing the serosal side while preventing immersion of the villi. After eight days, up to 33% of the samples displayed no histological abnormalities. Numerical simulation of the transport of oxygen and glucose provided technical solutions to improve the functionality of the microdevice.Show less >
Show more >Ex vivo explant culture models offer unique properties to study complex mechanisms underlying tissue growth, renewal, and disease. A major weakness is the short viability depending on the biopsy origin and preparation protocol. We describe an interphase microfluidic culture system to cultivate full thickness murine colon explants which keeps morphological structures of the tissue up to 192 h. The system was composed of a central well on top of a porous membrane supported by a microchannel structure. The microfluidic perfusion allowed bathing the serosal side while preventing immersion of the villi. After eight days, up to 33% of the samples displayed no histological abnormalities. Numerical simulation of the transport of oxygen and glucose provided technical solutions to improve the functionality of the microdevice.Show less >
Language :
Anglais
Popular science :
Non
Source :