Titre :

Connexin 30 locally controls actin cytoskeleton and mechanical remodeling in motile astrocytes

Auteur(s) :

Ghézali, Grégory [Auteur]
Ecole doctorale Cerveau Cognition et Comportement [Paris] [ED 158 - 3C]
Centre interdisciplinaire de recherche en biologie [CIRB]
Ribot, Jérôme [Auteur]
Centre interdisciplinaire de recherche en biologie [CIRB]
Curry, Nathan [Auteur]
Pillet, Laure‐elise [Auteur]
École Doctorale Bio Sorbonne Paris Cité [Paris] [ED562 - BioSPC]
Centre interdisciplinaire de recherche en biologie [CIRB]
Boutet-Porretta, Flora [Auteur]
Ecole doctorale Cerveau Cognition et Comportement [Paris] [ED 158 - 3C]
Centre interdisciplinaire de recherche en biologie [CIRB]
Mozheiko, Daria [Auteur]
Ecole doctorale Cerveau Cognition et Comportement [Paris] [ED 158 - 3C]
Centre interdisciplinaire de recherche en biologie [CIRB]
Calvo, Charles‐félix [Auteur]
Centre interdisciplinaire de recherche en biologie [CIRB]
Ezan, Pascal [Auteur]
Centre interdisciplinaire de recherche en biologie [CIRB]
Perfettini, Isabelle [Auteur]
Polarité cellulaire, Migration et Cancer - Cell Polarity, Migration and Cancer
Lecoin, Laure [Auteur]
Centre interdisciplinaire de recherche en biologie [CIRB]
Janel, Sébastien [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Zapata, Jonathan [Auteur]
Centre interdisciplinaire de recherche en biologie [CIRB]
Escartin, Carole [Auteur]
Laboratoire des Maladies Neurodégénératives - UMR 9199 [LMN]
Etienne-Manneville, Sandrine [Auteur]
Polarité cellulaire, Migration et Cancer - Cell Polarity, Migration and Cancer
Kaminski, Clemens [Auteur]
Rouach, Nathalie [Auteur correspondant]
Centre interdisciplinaire de recherche en biologie [CIRB]

Titre de la revue :

Glia

Pagination :

1915-1929

Éditeur :

Wiley

Date de publication :

2024

ISSN :

0894-1491

Mot(s)-clé(s) en anglais :

actin
astrocyte
connexin
motility
physical surface properties

Discipline(s) HAL :

Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Biologie cellulaire

Résumé en anglais : [en]

During brain maturation, astrocytes establish complex morphologies unveiling intense structural plasticity. Connexin 30 (Cx30), a gap‐junction channel‐forming protein expressed postnatally, dynamically regulates during ...
Lire la suite >During brain maturation, astrocytes establish complex morphologies unveiling intense structural plasticity. Connexin 30 (Cx30), a gap‐junction channel‐forming protein expressed postnatally, dynamically regulates during development astrocyte morphological properties by controlling ramification and extension of fine processes. However, the underlying mechanisms remain unexplored. Here, we found in vitro that Cx30 interacts with the actin cytoskeleton in astrocytes and inhibits its structural reorganization and dynamics during cell migration. This translates into an alteration of local physical surface properties, as assessed by correlative imaging using stimulated emission depletion (STED) super resolution imaging and atomic force microscopy (AFM). Specifically, Cx30 impaired astrocyte cell surface topology and cortical stiffness in motile astrocytes. As Cx30 alters actin organization, dynamics, and membrane physical properties, we assessed whether it controls astrocyte migration. We found that Cx30 reduced persistence and directionality of migrating astrocytes. Altogether, these data reveal Cx30 as a brake for astrocyte structural and mechanical plasticity.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Projet Européen :

Wiring synaptic circuits with astroglial connexins: mechanisms, dynamics and impact for critical period plasticity
Training, Research and Raising of Public Awareness in Cell Biology and Pathology of Neuroglia

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL