Titre :

CD69 Signaling in Eosinophils Induces IL-10 Production and Apoptosis via the Erk1/2 and JNK Pathways, Respectively

Auteur(s) :

Bui, Dan Van [Auteur]
Hanoï Medical University
Kansai University
Nguyen, Linh Manh [Auteur]
Kansai University
Kanda, Akira [Auteur]
Kansai University
Chu, Hanh Hong [Auteur]
Kansai University
Thi Le, Nhi Kieu [Auteur]
Kansai University
Yun, Yasutaka [Auteur]
Kansai University
Kobayashi, Yoshiki [Auteur]
Kansai University
Suzuki, Kensuke [Auteur]
Kansai University
Mitani, Akitoshi [Auteur]
Kansai University
Shimamura, Akihiro [Auteur]
Kansai University
Fukui, Kenta [Auteur]
Kansai University
Sawada, Shunsuke [Auteur]
Dombrowicz, David [Auteur]
Institut Pasteur de Lille
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U 1011 [RNMCD]
Iwai, Hiroshi [Auteur]
Kansai University

Titre de la revue :

Biomolecules

Pagination :

360

Éditeur :

MDPI

Date de publication :

2024-03-18

ISSN :

2218-273X

Mot(s)-clé(s) en anglais :

CD69
Erk1/2
IL-10
JNK
asthma
crosslinking
eosinophil
survival
type 2 inflammation

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Introduction: Eosinophils contribute to the pathogenesis of allergic diseases, including asthma, allergic rhinitis, and atopic dermatitis. We previously reported that human tissue eosinophils have high CD69 expression ...
Lire la suite >Introduction: Eosinophils contribute to the pathogenesis of allergic diseases, including asthma, allergic rhinitis, and atopic dermatitis. We previously reported that human tissue eosinophils have high CD69 expression compared to blood eosinophils, and its expression is correlated with disease severity and the number of infiltrated eosinophils. However, biological CD69 signaling activity in eosinophils remains unclear. Methods: CD69 expression on lung tissue eosinophils obtained from mice with ovalbumin-induced asthma was measured using flow cytometry. CD69 crosslinking was performed on eosinophils purified from the spleen of IL-5 transgenic mice to investigate CD69 signaling and its function in eosinophils. Then, qPCR, Western blot, enzyme-linked immunosorbent assay, and survival assay results were analyzed. Results: Surface CD69 expression on lung tissue eosinophils in the asthma mice model was 2.91% ± 0.76%, whereas no expression was detected in the healthy group. CD69-expressed eosinophils intrinsically have an upregulation of IL-10 mRNA expression. Moreover, CD69 crosslinking induced further pronounced IL-10 production and apoptosis; these responses were mediated via the Erk1/2 and JNK pathways, respectively. Conclusions: Our results suggested that CD69+ eosinophils play an immunoregulator role in type 2 inflammation, whereas activated tissue eosinophils contribute to the pathogenesis of asthma.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Récepteurs nucléaires, Maladies Cardiovasculaires et Diabète (RNMCD) - U1011

Source :

Harvested from HAL

Date de dépôt :

2025-01-22T22:50:53Z