Title :

Innovative Optimization of LDI-MS Porous Silicon Substrates Using Thermometer Ions

Author(s) :

Ferreira, Clara Whyte [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Université de Liège
Cabrera-Tejera, Bastien [Auteur]
Université de Liège
Leyh, Bernard [Auteur]
Université de Liège
Tuyaerts, Romain [Auteur]
Scheen, Gilles [Auteur]
Coffinier, Yannick [Auteur correspondant]
Nanostructures, nanoComponents & Molecules - IEMN [NCM - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Pauw, Edwin De [Auteur correspondant]
Université de Liège
Eppe, Gauthier [Auteur correspondant]
Université de Liège

HAL domain(s) :

Chimie
Sciences de l'ingénieur [physics]

English abstract : [en]

<div><p>This study presents a methodical procedure for optimizing laser desorption/ionization mass spectrometry (LDI-MS) supports using porous silicon (PSi) substrates. The approach involves the use of substituted ...
Show more ><div><p>This study presents a methodical procedure for optimizing laser desorption/ionization mass spectrometry (LDI-MS) supports using porous silicon (PSi) substrates. The approach involves the use of substituted benzyl-pyridinium salts (thermometer ions) to obtain one metric that assesses analyte fragmentation (the effective temperature of vibration). Porous silicon substrates were synthesized via electrochemical etching of p-type silicon wafers (10-20 mΩ⋅cm), with etching parameters adjusted to vary porosity while maintaining a layer thickness between 700 and 1200 nm. The results revealed that PSi substrates with 40-60% porosity achieved the lowest fragmentation levels. This finding was validated through the analysis of N-Acetyl glucosamine, a carbohydrate, which confirmed the effective temperature trend. Further analysis involving peptides, specifically P14R and a peptide mix (Peptide Calibration Standard II, Bruker), demonstrated that the optimized PSi substrates enabled the desorption and ionization of peptides with a maximum mass at m/z 2465, corresponding to ACTH clip 1-17. These results highlight the critical role of substrate porosity in minimizing analyte fragmentation and enhancing LDI-MS performance.</p></div>Show less >

Language :

Anglais

Collections :

• Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520

Source :

Harvested from HAL