Titre :

Voluntary exercise in mice triggers an anti-osteogenic and pro-tenogenic response in the ankle joint without affecting long bones

Auteur(s) :

Briolay, Anne [Auteur]
Institut de Chimie et Biochimie Moléculaires et Supramoléculaires [ICBMS]
Duboeuf, François [Auteur]
Physiopathologie, diagnostic et traitements des maladies osseuses / Pathophysiology, Diagnosis & Treatments of Bone Diseases [LYOS]
Delplace, Séverine [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Marrow Adiposity & Bone Lab - Adiposité Médullaire et Os - ULR 4490 [MABLab]
Brizuela, Leyre [Auteur]
Institut de Chimie et Biochimie Moléculaires et Supramoléculaires [ICBMS]
Peyruchaud, Olivier [Auteur]
Physiopathologie, diagnostic et traitements des maladies osseuses / Pathophysiology, Diagnosis & Treatments of Bone Diseases [LYOS]
Magne, David [Auteur]
Physiopathologie, diagnostic et traitements des maladies osseuses / Pathophysiology, Diagnosis & Treatments of Bone Diseases [LYOS]
Université Claude Bernard Lyon 1 [UCBL]
Bougault, Carole [Auteur]

Titre de la revue :

Bone Reports

Pagination :

101810

Éditeur :

Elsevier

Date de publication :

2024-12

ISSN :

2352-1872

Mot(s)-clé(s) en anglais :

Voluntary exercise
Ankle joint
Tendon
Bone
Ossification

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Biomechanical stimulation is proposed to occupy a central place in joint homeostasis, but the precise contribution of exercise remains elusive. We aimed to characterize in vivo the impact of mechanical stimulation on ...
Lire la suite >Biomechanical stimulation is proposed to occupy a central place in joint homeostasis, but the precise contribution of exercise remains elusive. We aimed to characterize in vivo the impact of mechanical stimulation on thecell-controlled regulation of ossification within the ankles of healthy mice undergoing mild physical activity.DBA/1 male mice were subjected to voluntary running exercise for two weeks, and compared to mice housed instandard conditions (n = 20 per group). Free access to activity wheels resulted in a running exercise of 5.5 ± 0.8km/day at 14.5 ± 0.5 m/min. Serum levels of alkaline phosphatase, IL-6, IL-8/Kc, IL-17a, and TNF-α weremeasured. No change in systemic inflammation was detected. The bone architecture of the femur and thecalcaneus was unchanged, as revealed by μCT and histology of the enthesis of the Achilles tendon. mRNAs wereextracted from femurs, tibias, and ankle joints before RT-qPCR analysis. The expression of the mechanosensitivegenes Sclerostin (Sost) and Periostin (Postn) was not impacted by the exercise in long bones. Oppositely, Sost andPostn levels were modulated by exercise in joints, and osteogenic markers (Col10a1, Runx2, Osx, and Dmp1) weredownregulated in the exercise group. In addition, the tenogenic markers Scx, Mkx, and Tnmd were upregulatedby exercise. Thus, voluntary exercise affected the phenotype of joint cells without impacting long bones. As geneexpression of Bmp2, Bmp4, and Id1 was also reduced in these cells, an off-regulation of BMP signaling could bepartly responsible for their mechanosensitive response. Running exercise seemed to preserve the tendon from itsprogressive ossification, as seen in numerous enthesopathies. This study paves the way to future experiments forinvestigating the effects of mechanical stimulation in various mouse models.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Marrow Adiposity & Bone Lab (MABLab) - ULR 4490

Source :

Harvested from HAL