Title :

Identification of cell wall proteins in the flax ( Linum usitatissimum ) stem

Author(s) :

Day, Arnaud [Auteur]
Fénart, Stéphane [Auteur]
Neutelings, Godfrey [Auteur]
Hawkins, Simon [Auteur]
Rolando, Christian [Auteur]
Tokarski, Caroline [Auteur]

Journal title :

Proteomics

Volume number :

13

Pages :

812-825

Publisher :

Wiley-VCH Verlag

Publication date :

2013-03

ISSN :

16159853

Keyword(s) :

Flax
Plant proteomics
PROTEOMICS
L.

English keyword(s) :

Cell wall
FT-ICR high resolution MS
PLANT GLYCOSIDE HYDROLASES
GERMIN-LIKE PROTEIN
PECTIN METHYLESTERASE
ARABIDOPSIS-THALIANA
PHYSIOLOGICAL ROLES
ELONGATING CELLS
BAST FIBERS
PEROXIDASE

HAL domain(s) :

Chimie/Chimie théorique et/ou physique
Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Biologie végétale

English abstract : [en]

Sequential salt (CaCl2, LiCl) extractions were used to obtain fractions enriched in cell wall proteins (CWPs) from the stem of 60-day-old flax (Linum usitatissimum) plants. High-resolution FT-ICR MS analysis and the use ...
Show more >Sequential salt (CaCl2, LiCl) extractions were used to obtain fractions enriched in cell wall proteins (CWPs) from the stem of 60-day-old flax (Linum usitatissimum) plants. High-resolution FT-ICR MS analysis and the use of recently published genomic data allowed the identification of 11912 peptides corresponding to a total of 1418 different proteins. Subcellular localization using TargetP, Predotar, and WoLF PSORT led to the identification of 152 putative flax CWPs that were classified into nine different functional classes previously established for Arabidopsis thaliana. Examination of different functional classes revealed the presence of a number of proteins known to be involved in, or potentially involved in cell-wall metabolism in plants. The flax stem cell wall proteome was also compared with transcriptomic data previously obtained on comparable samples. This study represents a major contribution to the identification of CWPs in flax and will lead to a better understanding of cell wall biology in this species.Show less >

Language :

Anglais

Popular science :

Non

Administrative institution(s) :

CNRS
Université de Lille

Collections :

• Miniaturisation pour la Synthèse, l'Analyse et la Protéomique (MSAP) - USR 3290
• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Fibres végétales

Submission date :

2020-02-12T15:45:16Z