Title :

Activity of enzymes immobilized on plasma treated polyester

Author(s) :

Mohamed, Arfaoui [Auteur]
Université de Lille
École nationale supérieure des arts et industries textiles [ENSAIT]
Nemeshwaree, Behary [Auteur]
Université de Lille
École nationale supérieure des arts et industries textiles [ENSAIT]
Brigitte, Mutel [Auteur]
Université de Lille
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Anne, Perwuelz [Auteur]
Université de Lille
École nationale supérieure des arts et industries textiles [ENSAIT]
Kalim, Belhacene [Auteur]
Laboratoire des Procédés Biologiques Génie Enzymatique et Microbien [PROBIOGEM]
Université de Lille
Institut Charles Viollette (ICV) - ULR 7394 [ICV]
Dhulster, Pascal [Auteur]
Laboratoire des Procédés Biologiques Génie Enzymatique et Microbien [PROBIOGEM]
Université de Lille
Institut Charles Viollette (ICV) - ULR 7394
Mamede, Anne-Sophie [Auteur]
Unité de Catalyse et Chimie du Solide - UMR 8181 [UCCS]
Université de Lille
Froidevaux, Rénato [Auteur]
Laboratoire des Procédés Biologiques Génie Enzymatique et Microbien [PROBIOGEM]
Université de Lille
Institut Charles Viollette (ICV) - ULR 7394 [ICV]

Journal title :

Journal of Molecular Catalysis B. Enzymatic

Volume number :

134

Pages :

261-272

Publication date :

2016-12

HAL domain(s) :

Chimie/Catalyse

English abstract : [en]

The aim of this study was to investigate the effect of 3 different surface plasma treatments on the immobilization of β-galactosidase on a fibrous PET nonwoven membrane. Two methods 1. Entrapment in a thin calcium alginate ...
Show more >The aim of this study was to investigate the effect of 3 different surface plasma treatments on the immobilization of β-galactosidase on a fibrous PET nonwoven membrane. Two methods 1. Entrapment in a thin calcium alginate coating and 2. Direct sorption, were used to immobilize the enzyme. The three different plasma treatments for surface activation of PET nonwovens were: 1. Air atmospheric DBD plasma, 2. Cold remote plasma-CRP with 100% N2 and 3.- CRP with a mixture of N2/O2 gases. Plasma treatment of the PET fiber surface increased the quantity of immobilized enzyme using the entrapment method, and the degree of alginate film cross-linking highly influenced the enzyme activity. Highest enzyme activity was reached for the PET treated with air atmospheric plasma and cross-linked with 0.25 g/l of CaCl2. With the direct sorption method, greater amounts of enzyme were immobilized as compared to the entrapment method, but a considerable proportion of enzyme lost their catalytic activity. Only with the CRP N2/O2 plasma treatment, up to 90% of sorbed enzyme maintained their activity. Reusability study showed that for the optimized entrapment method, a progressive decrease in activity was observed after each use cycle. With the optimized sorption method using N2/O2 CRP plasma, no decrease in enzyme activity was detected, and the immobilized enzyme could be used over more than 15 cycles.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

ENSCL
CNRS
INRA
ISA
Centrale Lille
Univ. Artois
Univ. Littoral Côte d’Opale
Université de Lille

Collections :

• BioEcoAgro - UMR-T 1158
• Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181

Research team(s) :

Matériaux pour la catalyse (MATCAT)

Submission date :

2019-09-25T14:38:11Z
2024-04-22T14:39:37Z