Titre :

The mirna-targeted transcriptome of porcine alveolar macrophages upon infection with porcine reproductive and respiratory syndrome virus

Auteur(s) :

Dhorne-Pollet, Sophie [Auteur]
Crisci, Elisa [Auteur]
Mach, Nuria [Auteur]
Renson, Patricia [Auteur]
Jaffrezic, Florence [Auteur]
Marot, Guillemette [Auteur]
Evaluation des technologies de santé et des pratiques médicales - ULR 2694 [METRICS]
Maroilley, Tatiana [Auteur]
Moroldo, Marco [Auteur]
Lecardonnel, Jerome [Auteur]
Blanc, Fany [Auteur]
Bertho, Nicolas [Auteur]
Bourry, Olivier [Auteur]
Giuffra, Elisabetta [Auteur]

Titre de la revue :

Scientific reports

Nom court de la revue :

Sci Rep

Numéro :

9

Pagination :

3160

Date de publication :

2019-02-28

ISSN :

2045-2322

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Host miRNAs are known to modulate the cell response to virus infections. We characterized the miRNA-targeted transcriptome of porcine alveolar macrophages (PAMs) at early times after infection with a subtype 1.1 strain of ...
Lire la suite >Host miRNAs are known to modulate the cell response to virus infections. We characterized the miRNA-targeted transcriptome of porcine alveolar macrophages (PAMs) at early times after infection with a subtype 1.1 strain of PRRSV (Porcine Reproductive and Respiratory Syndrome Virus). We performed the immunoprecipitation of RISC (RNA-induced Silencing Complex) followed by microarray analysis of the RISC-bound miRNA targets (RIP-Chip) to evaluate the relative enrichment or depletion of expressed genes in RISC. The miRNA-mediated regulation occurred early after PRRSV infection and decreased fast (1,241 and 141 RISC-bound genes at 7 h and 10 h post-infection, respectively); it affected several cell functions with evidence of miRNA buffering of upregulated interferon-related genes. Eight miRNAs were highly enriched in RISC of both control and infected cells with no evidence of differential expression. Although miR-335-5p was the miRNA with most predicted targets among enriched RISC-bound genes, no effects on surface markers, cytokine expression and PRRSV replication were detected upon miR-335-5p mimics of primary PAMs. Our results do not point to specific miRNA-driven mechanisms regulating the early response to infection with this PRRSV 1.1 strain and indicate that the miRNome expressed by steady-state PAMs reacts promptly to counterbalance PRRSV infection by a pervasive modulation of host functions.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

CHU Lille
Université de Lille

Collections :

• METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694

Date de dépôt :

2019-12-09T16:48:19Z