Title :

Comparison of two commercial quantitative PCR assays for EBV DNA detection and their correlation with the first WHO International Standard for EBV

Author(s) :

Engelmann, Ilka [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Alidjinou, Enagnon Kazali [Auteur]
Laboratoire de virologie - ULR 3610
Lazrek, Mouna [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Pouillaude, Jean-Marie [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Ogiez, Judith [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Rose, François [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Duhamel, Alain [Auteur]
Evaluation des technologies de santé et des pratiques médicales - ULR 2694 [METRICS]
Dewilde, Anny [Auteur]
Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie]
Hober, Didier [Auteur]
215293|||Laboratoire de Virologie - ULR 3610 [Laboratoire de Virologie] (VALID)

Journal title :

Journal of Medical Microbiology

Abbreviated title :

J. Med. Microbiol.

Volume number :

67

Publisher :

Society for General Microbiology

Publication date :

2018-02-26

ISSN :

1473-5644

English keyword(s) :

calibration
viral load
molecular testing
diagnostics

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Purpose. There are few data on the performance of automated Epstein–Barr virus (EBV) PCR assays. This study compared EBV quantification for the kPCR PLX EBV DNA (kPCR; Siemens, France) and the EBV R-gene (R-gene; Argene, ...
Show more >Purpose. There are few data on the performance of automated Epstein–Barr virus (EBV) PCR assays. This study compared EBV quantification for the kPCR PLX EBV DNA (kPCR; Siemens, France) and the EBV R-gene (R-gene; Argene, Biomerieux, France) assays and their correlation with the World Health Organization (WHO) standard. Methodology. WHO International Standard for EBV (WHO standard) dilution panels in different matrices were submitted to nucleic acid extraction with Versant kPCR Molecular Systems SP followed by the kPCR assay, or to nucleic acid extraction with the MagNA Pure LC System or NucliSENS easyMag followed by the R-gene assay. Seventy-four clinical specimens were tested in both assays. Bland–Altman analysis and linear regression analysis were performed. Results. The correlation between the WHO standard diluted in different matrices and the R-gene and kPCR assays was good (R2 >0.96 and R2 >0.92, respectively). A matrix effect was observed. The correlation of quantitative results between both assays yielded a coefficient of determination R2 higher than 0.74. The quantification differences were within one log10 of the averaged results for 34 of the 38 specimens (89 %). Calibration to the WHO standard did not increase the comparability of quantitative results. Conclusions. The quantitative results of both assays showed reasonable correlation with each other and a good correlation with the WHO standard.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Université de Lille

Collections :

• Laboratoire de virologie - ULR 3610
• METRICS : Evaluation des technologies de santé et des pratiques médicales - ULR 2694

Submission date :

2020-02-11T09:07:36Z
2024-02-23T10:03:36Z