Title :

Bacteroides thetaiotaomicron and Faecalibacterium prausnitzii influence the production of mucus glycans and the development of goblet cells in the colonic epithelium of a gnotobiotic model rodent

Author(s) :

Wrzosek, Laura [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Miquel, Sylvie [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Noordine, Marie-Louise [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Bouet, Stephan [Auteur]
Génétique Animale et Biologie Intégrative [GABI]
Joncquel, Marie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Robert, Véronique [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Philippe, Catherine [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Bridonneau, Chantal [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Cherbuy, Claire [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Masselot, Catherine [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Langella, Philippe [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Thomas, Muriel [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]

Journal title :

BMC Biology

Volume number :

11

Pages :

61

Publication date :

2013-05-21

ISSN :

1741-7007

English keyword(s) :

Germ-free rat
Goblet cell
klf4
Short chain fatty acid
mucin o-glycosylation

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Background: The intestinal mucus layer plays a key role in the maintenance of host-microbiota homeostasis. To document the crosstalk between the host and microbiota, we used gnotobiotic models to study the influence of two ...
Show more >Background: The intestinal mucus layer plays a key role in the maintenance of host-microbiota homeostasis. To document the crosstalk between the host and microbiota, we used gnotobiotic models to study the influence of two major commensal bacteria, Bacteroides thetaiotaomicron and Faecalibacterium prausnitzii, on this intestinal mucus layer. B. thetaiotaomicron is known to use polysaccharides from mucus, but its effect on goblet cells has not been addressed so far. F. prausnitzii is of particular physiological importance because it can be considered as a sensor and a marker of human health. We determined whether B. thetaiotaomicron affected goblet cell differentiation, mucin synthesis and glycosylation in the colonic epithelium. We then investigated how F. prausnitzii influenced the colonic epithelial responses to B. thetaiotaomicron.[br/] Results: B. thetaiotaomicron, an acetate producer, increased goblet cell differentiation, expression of mucus-related genes and the ratio of sialylated to sulfated mucins in mono-associated rats. B. thetaiotaomicron, therefore, stimulates the secretory lineage, favoring mucus production. When B. thetaiotaomicron was associated with F. prausnitzii, an acetate consumer and a butyrate producer, the effects on goblet cells and mucin glycosylation were diminished. F. prausnitzii, by attenuating the effects of B. thetaiotaomicron on mucus, may help the epithelium to maintain appropriate proportions of different cell types of the secretory lineage. Using a mucus-producing cell line, we showed that acetate up-regulated KLF4, a transcription factor involved in goblet cell differentiation.[br/] Conclusions: B. thetaiotaomicron and F. prausnitzii, which are metabolically complementary, modulate, in vivo, the intestinal mucus barrier by modifying goblet cells and mucin glycosylation. Our study reveals the importance of the balance between two main commensal bacteria in maintaining colonic epithelial homeostasis via their respective effects on mucus.Show less >

Language :

Anglais

Audience :

Non spécifiée

Administrative institution(s) :

CNRS
Université de Lille

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Génétique des enveloppes bactériennes

Submission date :

2020-02-12T15:44:35Z
2021-04-16T12:28:02Z