Title :

Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species

Author(s) :

Ambruosi, Barbara [Auteur]
Physiologie de la reproduction et des comportements [Nouzilly] [PRC]
Accogli, Gianluca [Auteur]
Università degli studi di Bari Aldo Moro = University of Bari Aldo Moro [UNIBA]
Douet, Cécile [Auteur]
Physiologie de la reproduction et des comportements [Nouzilly] [PRC]
Canepa, Sylvie [Auteur]
Physiologie de la reproduction et des comportements [Nouzilly] [PRC]
Pascal, Géraldine [Auteur]
Physiologie de la reproduction et des comportements [Nouzilly] [PRC]
Monget, Philippe [Auteur]
Physiologie de la reproduction et des comportements [Nouzilly] [PRC]
Moros, Carla [Auteur]
Universidad de Murcia
Holmskov, Uffe [Auteur]
University of Southern Denmark [SDU]
Mollenhauer, Jan [Auteur]
University of Southern Denmark [SDU]
Masselot, Catherine [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Vidal, Olivier [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Desantis, Salvatore [Auteur]
Università degli studi di Bari Aldo Moro = University of Bari Aldo Moro [UNIBA]
Goudet, Ghylène [Auteur]
Physiologie de la reproduction et des comportements [Nouzilly] [PRC]

Journal title :

Reproduction

Volume number :

146

Pages :

119-133

Publication date :

2013-08-01

ISSN :

1470-1626, 1741-7899

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Oviductal environment affects preparation of gametes for fertilization, fertilization itself, and subsequent embryonic development. Theaim of this study was to evaluate the effect of oviductal fluid and the possible ...
Show more >Oviductal environment affects preparation of gametes for fertilization, fertilization itself, and subsequent embryonic development. Theaim of this study was to evaluate the effect of oviductal fluid and the possible involvement of deleted in malignant brain tumor 1 (DMBT1)on IVF in porcine and equine species that represent divergent IVF models. We first performed IVF after pre-incubation of oocytes withor without oviductal fluid supplemented or not with antibodies directed against DMBT1. We showed that oviductal fluid induces anincrease in the monospermic fertilization rate and that this effect is canceled by the addition of antibodies, in both porcine and equinespecies. Moreover, pre-incubation of oocytes with recombinant DMBT1 induces an increase in the monospermic fertilization rate in thepig, confirming an involvement of DMBT1 in the fertilization process. The presence of DMBT1 in the oviduct at different stages of theestrus cycle was shown by western blot and confirmed by immunohistochemical analysis of ampulla and isthmus regions. The presence ofDMBT1 in cumulus–oocyte complexes was shown by western blot analysis, and the localization of DMBT1 in the zona pellucida andcytoplasm of equine and porcine oocytes was observed using immunofluorescence analysis and confocal microscopy. Moreover, weshowed an interaction between DMBT1 and porcine spermatozoa using surface plasmon resonance studies. Finally, a bioinformatic andphylogenetic analysis allowed us to identify the DMBT1 protein as well as a DMBT1-like protein in several mammals. Our results stronglysuggest an important role of DMBT1 in the process of fertilization.Show less >

Language :

Anglais

Audience :

Non spécifiée

Administrative institution(s) :

CNRS
Université de Lille

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Génétique des enveloppes bactériennes

Submission date :

2020-02-12T15:45:03Z
2021-05-20T07:47:33Z