Intra-platform comparison of 25-mer and ...
Type de document :
Article dans une revue scientifique
DOI :
URL permanente :
Titre :
Intra-platform comparison of 25-mer and 60-mer oligonucleotide Nimblegen DNA microarrays
Auteur(s) :
Fénart, Stéphane [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
CHABI, MALIKA [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Gallina, Sophie [Auteur]
Génétique et évolution des populations végétales [GEPV]
Huis, Rudy [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Neutelings, Godfrey [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Riviere, Nathalie [Auteur]
Biogemma
Thomasset, Brigitte [Auteur]
Génie Enzymatique et Cellulaire [GEC]
Hawkins, Simon [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Lucau, Anca [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
CHABI, MALIKA [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Gallina, Sophie [Auteur]
Génétique et évolution des populations végétales [GEPV]
Huis, Rudy [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Neutelings, Godfrey [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Riviere, Nathalie [Auteur]
Biogemma
Thomasset, Brigitte [Auteur]
Génie Enzymatique et Cellulaire [GEC]
Hawkins, Simon [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Lucau, Anca [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Titre de la revue :
BMC research notes
Numéro :
6
Pagination :
43
Date de publication :
2013
ISSN :
1756-0500
Mot(s)-clé(s) en anglais :
Nimblegen
DNA arrays
Gene expression
DNA arrays
Gene expression
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
Background: We performed a Nimblegen intra-platform microarray comparison by assessing two categories of flax target probes (short 25-mers oligonucleotides and long 60-mers oligonucleotides) in identical conditions of ...
Lire la suite >Background: We performed a Nimblegen intra-platform microarray comparison by assessing two categories of flax target probes (short 25-mers oligonucleotides and long 60-mers oligonucleotides) in identical conditions of target production, design, labelling, hybridization, image analyses, and data filtering. We compared technical parameters of array hybridizations, precision and accuracy as well as specific gene expression profiles. Results: Comparison of the hybridization quality, precision and accuracy of expression measurements, as well as an interpretation of differential gene expression in flax tissues were performed. Both array types yielded reproducible, accurate and comparable data that are coherent for expression measurements and identification of differentially expressed genes. 60-mers arrays gave higher hybridization efficiencies and therefore were more sensitive allowing the detection of a higher number of unigenes involved in the same biological process and/or belonging to the same multigene family. Conclusion: The two flax arrays provide a good resolution of expressed functions; however the 60-mers arrays are more sensitive and provide a more in-depth coverage of candidate genes potentially involved in different biological processes.Lire moins >
Lire la suite >Background: We performed a Nimblegen intra-platform microarray comparison by assessing two categories of flax target probes (short 25-mers oligonucleotides and long 60-mers oligonucleotides) in identical conditions of target production, design, labelling, hybridization, image analyses, and data filtering. We compared technical parameters of array hybridizations, precision and accuracy as well as specific gene expression profiles. Results: Comparison of the hybridization quality, precision and accuracy of expression measurements, as well as an interpretation of differential gene expression in flax tissues were performed. Both array types yielded reproducible, accurate and comparable data that are coherent for expression measurements and identification of differentially expressed genes. 60-mers arrays gave higher hybridization efficiencies and therefore were more sensitive allowing the detection of a higher number of unigenes involved in the same biological process and/or belonging to the same multigene family. Conclusion: The two flax arrays provide a good resolution of expressed functions; however the 60-mers arrays are more sensitive and provide a more in-depth coverage of candidate genes potentially involved in different biological processes.Lire moins >
Langue :
Anglais
Audience :
Non spécifiée
Établissement(s) :
CNRS
Université de Lille
Université de Lille
Collections :
Équipe(s) de recherche :
Fibres végétales
Date de dépôt :
2020-02-12T15:45:17Z
2021-03-17T08:32:21Z
2021-06-08T13:15:35Z
2021-03-17T08:32:21Z
2021-06-08T13:15:35Z
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