Titre :

Microsatellite loci to recognize species for the cheese starter and contaminating strains associated with cheese manufacturing

Auteur(s) :

Giraud, Frédéric [Auteur]
Méthodes et outils pour l'Interaction à gestes [MINT2]
Giraud, Tatiana [Auteur]
AgroParisTech
Université Paris Saclay (COmUE)
Ecologie Systématique et Evolution [ESE]
Centre National de la Recherche Scientifique [CNRS]
Université Paris-Sud - Paris 11 [UP11]
Aguileta, Gabriela [Auteur]
Centre National de la Recherche Scientifique [CNRS]
Fournier, Elisabeth [Auteur]
Samson, Robert [Auteur]
Cruaud, Corinne [Auteur]
Genoscope - Centre national de séquençage [Evry] [GENOSCOPE]
Lacoste, Sandrine [Auteur]
Institut de Systématique, Evolution, Biodiversité [ISYEB]
Ropars, Jeanne [Auteur]
Centre National de la Recherche Scientifique [CNRS]
Tellier, Aurelien [Auteur]
Hochschule Weihenstephan-Triesdorf [Freising] [HSWT]
Dupont, Joëlle [Auteur]
Institut de Systématique, Evolution, Biodiversité [ISYEB]

Titre de la revue :

International journal of food microbiology

Pagination :

204-213

Éditeur :

Elsevier

Date de publication :

2010-02

ISSN :

0168-1605

Mot(s)-clé(s) :

CONTAMINANT
DOMESTICATION

Mot(s)-clé(s) en anglais :

PC4 MARKER
MULTIGENIC PHYLOGENY
PENICILLIUM
STARTER CULTURES

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Biodiversité/Evolution [q-bio.PE]

Résumé en anglais : [en]

We report the development of 17 microsatellite markers in the cheese fungi Penicillium camemberti and P. roqueforti, using an enrichment protocol. Polymorphism and cross-amplification were explored using 23 isolates of P. ...
Lire la suite >We report the development of 17 microsatellite markers in the cheese fungi Penicillium camemberti and P. roqueforti, using an enrichment protocol. Polymorphism and cross-amplification were explored using 23 isolates of P. camemberti, 26 isolates of P. roqueforti and 2 isolates of each of the A chtysogenum and P. nalgiovense species. used to produce meat fermented products. The markers appeared useful for differentiating species, both using their amplification sizes and the sequences of their flanking regions. The microsatellite locus PC4 was particularly suitable for distinguishing contaminant species closely related to P. camemberti and for clarifying the phylogenetic relationship of this species with its supposed ancestral form, P. commune. We analyzed 22 isolates from different culture collections assigned to the morphospecies P commune, most of them occurring as food spoilers, mainly from the cheese environment. None of them exhibited identical sequences with the ex-type isolate of the species P. commune. They were instead distributed into two other distinct lineages, corresponding to the old species P. fuscoglaucum and P. biforme, previously synonymised respectively with P. commune and P. camemberti. The ex-type isolate of P. commune was strictly identical to P. camemberti at all the loci examined. P. caseifulvum, a non toxinogenic species described as a new candidate for cheese fermentation, also exhibited sequences identical to P. camemberti. The microsatellite locus PC4 may therefore be considered as a useful candidate for the barcode of these economically important speciesLire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Commentaire :

UMR BGPI Equipe 5

Collections :

• Centre de Recherche en Informatique, Signal et Automatique de Lille (CRIStAL) - UMR 9189

Source :

Harvested from HAL