Titre :

Multi-loci diagnosis of acute lymphoblastic leukaemia with high-throughput sequencing and bioinformatics analysis

Auteur(s) :

Ferret, Yann [Auteur]
Service d'Hématologie Cellulaire [Lille]
Caillault, Aurélie [Auteur]
Service d'Hématologie Cellulaire [Lille]
Sebda, Shéhérazade [Auteur]
Plateforme de génomique fonctionnelle et structurelle [Lille]
Duez, Marc [Auteur]
Site de Recherche Intégrée en Cancérologie [SIRIC-ONCOLille]
School of Social and Community Medicine [Bristol]
Grardel, Nathalie [Auteur]
Service d'Hématologie Cellulaire [Lille]
Duployez, Nicolas [Auteur]
Service d'Hématologie Cellulaire [Lille]
Villenet, Céline [Auteur]
Plateforme de génomique fonctionnelle et structurelle [Lille]
Figeac, Martin [Auteur]
Plateforme de génomique fonctionnelle et structurelle [Lille]
Preudhomme, Claude [Auteur]
Service d'Hématologie Cellulaire [Lille]
Salson, Mikael [Auteur]
Centre de Recherche en Informatique, Signal et Automatique de Lille - UMR 9189 [CRIStAL]
Bioinformatics and Sequence Analysis [BONSAI]
Giraud, Mathieu [Auteur]
Centre de Recherche en Informatique, Signal et Automatique de Lille - UMR 9189 [CRIStAL]
Bioinformatics and Sequence Analysis [BONSAI]

Titre de la revue :

British Journal of Haematology

Pagination :

413-420

Éditeur :

Wiley

Date de publication :

2016

ISSN :

0007-1048

Mot(s)-clé(s) en anglais :

Acute Lymphoblastic Leukaemia (ALL)
Bioinformatics
Diagnosis
High-Throughput Sequencing (HTS)
Immune Repertoire Sequencing (Rep-Seq)

Discipline(s) HAL :

Informatique [cs]/Bio-informatique [q-bio.QM]
Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Hématologie
Sciences du Vivant [q-bio]/Immunologie

Résumé en anglais : [en]

High-throughput sequencing (HTS) is considered a technical revolution that has improved our knowledge of lymphoid and autoimmune diseases, changing our approach to leukaemia both at diagnosis and during follow-up. As part ...
Lire la suite >High-throughput sequencing (HTS) is considered a technical revolution that has improved our knowledge of lymphoid and autoimmune diseases, changing our approach to leukaemia both at diagnosis and during follow-up. As part of an immunoglobulin/T cell receptor-based minimal residual disease (MRD) assessment of acute lymphoblastic leukaemia patients, we assessed the performance and feasibility of the replacement of the first steps of the approach based on DNA isolation and Sanger sequencing, using a HTS protocol combined with bioinformatics analysis and visualization using the Vidjil software. We prospectively analysed the diagnostic and relapse samples of 34 paediatric patients, thus identifying 125 leukaemic clones with recombinations on multiple loci (TRG, TRD, IGH and IGK), including Dd2/Dd3 and Intron/KDE rearrangements. Sequencing failures were halved (14% vs. 34%, P = 0.0007), enabling more patients to be monitored. Furthermore, more markers per patient could be monitored, reducing the probability of false negative MRD results. The whole analysis, from sample receipt to clinical validation, was shorter than our current diagnostic protocol, with equal resources. V(D)J recombination was successfully assigned by the software, even for unusual recombinations. This study emphasizes the progress that HTS with adapted bioinformatics tools can bring to the diagnosis of leukaemia patients.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Centre de Recherche en Informatique, Signal et Automatique de Lille (CRIStAL) - UMR 9189

Source :

Harvested from HAL