Titre :

Hepatitis E Virus (HEV) Open Reading Frame 2 Antigen Kinetics in Human-Liver Chimeric Mice and Its Impact on HEV Diagnosis

Auteur(s) :

Sayed, Ibrahim [Auteur]
Assiut University
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Verhoye, Lieven [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Montpellier, Claire [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Legrand-Abravanel, Florence [Auteur]
Laboratoire Virologie [CHU Toulouse]
Centre de Physiopathologie Toulouse Purpan [CPTP]
Cocquerel, Laurence [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Meuleman, Philip [Auteur]
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Izopet, Jacques [Auteur]
Centre de Physiopathologie Toulouse Purpan [CPTP]
Laboratoire Virologie [CHU Toulouse]

Titre de la revue :

Journal of Infectious Diseases

Pagination :

811-819

Éditeur :

Oxford University Press

Date de publication :

2019

ISSN :

0022-1899

Mot(s)-clé(s) en anglais :

ribavirin therapy
HEV Ag
humanized mice
ORF2
diagnosis

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Immunologie
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Maladies infectieuses
Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Background: Hepatitis E virus infection (HEV) is an emerging problem in developed countries. Diagnosis of HEV infection is based on the detection of HEV-specific antibodies, viral RNA and/or antigens (Ag). Humanized mice ...
Lire la suite >Background: Hepatitis E virus infection (HEV) is an emerging problem in developed countries. Diagnosis of HEV infection is based on the detection of HEV-specific antibodies, viral RNA and/or antigens (Ag). Humanized mice were previously reported as a model for the study of HEV infection, but published data was focused on the quantification of viral RNA. However, the kinetics of HEV Ag expression during the course of infection remains poorly understood.Methods: Plasma and fecal suspensions from HEV infected and ribavirin-treated humanized mice were analyzed using HEV antigen ELISA, RT-qPCR, density gradient and Western blotting.Result: ORF2 Ag was detected in both plasma and stool of HEV infected mice, and increased overtime. Contrary to HEV RNA, ORF2 Ag levels were higher in mouse plasma than in stool. Interestingly, ORF2 was detected in plasma of mice that were RNA negative in plasma but RNA positive in stool; and after viral clearance by ribavirin. Plasma density gradient analysis revealed the presence of the non-infectious glycosylated form of ORF2.Conclusion: ORF2 Ag can be used as a marker of active HEV infection and the assessment of antiviral therapy, especially when fecal samples are not available or molecular diagnostic tests are not accessible.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL