Titre :

Morphology and Molecular Composition of Purified Bovine Viral Diarrhea Virus Envelope

Auteur(s) :

Callens, Nathalie [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Brügger, Britta [Auteur]
Universität Heidelberg [Heidelberg] = Heidelberg University
Bonnafous, Pierre [Auteur]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Drobecq, Herve [Auteur]
Institut de biologie de Lille - UMS 3702 [IBL]
Gerl, Mathias [Auteur]
Universität Heidelberg [Heidelberg] = Heidelberg University
Krey, Thomas [Auteur]
Virologie Structurale - Structural Virology
Roman-Sosa, Gleyder [Auteur]
Friedrich-Loeffler-Institut [FLI]
Rümenapf, Till [Auteur]
Lambert, Olivier [Auteur]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Dubuisson, Jean [Auteur]
Assemblage et réplication du virus de l'hépatite C [ARVHC]
Rouillé, Yves [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]

Titre de la revue :

PLOS Pathogens

Pagination :

e1005476

Éditeur :

Public Library of Science

Date de publication :

2016

ISSN :

1553-7366

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
Sciences du Vivant [q-bio]/Biologie cellulaire/Organisation et fonctions cellulaires [q-bio.SC]

Résumé en anglais : [en]

The family Flaviviridae includes viruses that have different virion structures and morphogen-esis mechanisms. Most cellular and molecular studies have been so far performed with viruses of the Hepacivirus and Flavivirus ...
Lire la suite >The family Flaviviridae includes viruses that have different virion structures and morphogen-esis mechanisms. Most cellular and molecular studies have been so far performed with viruses of the Hepacivirus and Flavivirus genera. Here, we studied bovine viral diarrhea virus (BVDV), a member of the Pestivirus genus. We set up a method to purify BVDV virions and analyzed their morphology by electron microscopy and their protein and lipid composition by mass spectrometry. Cryo-electron microscopy showed near spherical viral particles displaying an electron-dense capsid surrounded by a phospholipid bilayer with no visible spikes. Most particles had a diameter of 50 nm and about 2% were larger with a diameter of up to 65 nm, suggesting some size flexibility during BVDV morphogenesis. Morphological and biochemical data suggested a low envelope glycoprotein content of BVDV particles, E1 and E2 being apparently less abundant than E rns. Lipid content of BVDV particles displayed a ~2.3 to 3.5-fold enrichment in cholesterol, sphingomyelin and hexosyl-ceramide, concomi-tant with a 1.5 to 5-fold reduction of all glycerophospholipid classes, as compared to lipid content of MDBK cells. Although BVDV buds in the endoplasmic reticulum, its lipid content differs from a typical endoplasmic reticulum membrane composition. This suggests that BVDV morphogenesis includes a mechanism of lipid sorting. Functional analyses confirmed the importance of cholesterol and sphingomyelin for BVDV entry. Surprisingly, despite a high cholesterol and sphingolipid content of BVDV envelope, E2 was not found in detergent-resistant membranes. Our results indicate that there are differences between the structure and molecular composition of viral particles of Flaviviruses, Pestiviruses and Hepaciviruses within the Flaviviridae family. PLOS Pathogens |Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL