Titre :

Role of Hydrophobins in Aspergillus fumigatus

Auteur(s) :

Valsecchi, Isabel [Auteur]
Résonance Magnétique Nucléaire des Biomolécules
Aspergillus
Dupres, Vincent [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Stephen-Victor, Emmanuel [Auteur]
Centre de Recherche des Cordeliers [CRC]
Guijarro, J. Iñaki [Auteur]
Résonance Magnétique Nucléaire des Biomolécules
Gibbons, John [Auteur]
Clark University
Beau, Rémi [Auteur]
Aspergillus
Bayry, Jagadeesh [Auteur]
Centre de Recherche des Cordeliers [CRC]
Coppee, Jean-Yves [Auteur]
Transcriptome et Epigénome [PF2]
Lafont, Frank [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Latgé, Jean-Paul [Auteur]
Aspergillus
Beauvais, Anne [Auteur correspondant]
Aspergillus

Titre de la revue :

JOURNAL OF FUNGI

Pagination :

2

Éditeur :

MDPI

Date de publication :

2017-12-24

ISSN :

2309-608X

Mot(s)-clé(s) en anglais :

rodlet
cell wall
hydrophobin
Aspergillus

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Mycologie

Résumé en anglais : [en]

Resistance of Aspergillus fumigatus conidia to desiccation and their capacity to reach the alveoli are partly due to the presence of a hydrophobic layer composed of a protein from the hydrophobin family, called RodA, which ...
Lire la suite >Resistance of Aspergillus fumigatus conidia to desiccation and their capacity to reach the alveoli are partly due to the presence of a hydrophobic layer composed of a protein from the hydrophobin family, called RodA, which covers the conidial surface. In A. fumigatus there are seven hydrophobins (RodA–RodG) belonging to class I and III. Most of them have never been studied. We constructed single and multiple hydrophobin-deletion mutants until the generation of a hydrophobin-free mutant. The phenotype, immunogenicity, and virulence of the mutants were studied. RODA is the most expressed hydrophobin in sporulating cultures, whereas RODB is upregulated in biofilm conditions and in vivo. Only RodA, however, is responsible for rodlet formation, sporulation, conidial hydrophobicity, resistance to physical insult or anionic dyes, and immunological inertia of the conidia. None of the hydrophobin plays a role in biofilm formation or its hydrophobicity. RodA is the only needed hydrophobin in A. fumigatus, conditioning the structure, permeability, hydrophobicity, and immune-inertia of the cell wall surface in conidia. Moreover, the defect of rodlets on the conidial cell wall surface impacts on the drug sensitivity of the fungus.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Projet ANR :

Amyloïdes fonctionnels formés par les hydrophobines du pathogène fongique Aspergillus fumigatus

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL