Title :

A TCRβ Repertoire Signature Can Predict Experimental Cerebral Malaria

Author(s) :

Mariotti-Ferrandiz, Encarnita [Auteur]
Immunologie - Immunopathologie - Immunothérapie [I3]
Immunophysiopathologie Infectieuse
Département d'Immunologie - Department of Immunology
Pham, Hang-Phuong [Auteur]
CHU Pitié-Salpêtrière [AP-HP]
Immunologie - Immunopathologie - Immunothérapie [I3]
Dulauroy, Sophie [Auteur]
Immunophysiopathologie Infectieuse
Département d'Immunologie - Department of Immunology
Développement des Tissus Lymphoïdes
Gorgette, Olivier [Auteur]
Microscopie ultrastructurale (plate-forme)
Immunophysiopathologie Infectieuse
Département d'Immunologie - Department of Immunology
Klatzmann, David [Auteur]
Immunologie - Immunopathologie - Immunothérapie [I3]
Cazenave, Pierre-André [Auteur]
Immunophysiopathologie Infectieuse
Département d'Immunologie - Department of Immunology
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Pied, Sylviane [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Immunophysiopathologie Infectieuse
Département d'Immunologie - Department of Immunology
Six, Adrien [Auteur correspondant]
Immunologie - Immunopathologie - Immunothérapie [I3]
Immunophysiopathologie Infectieuse
Département d'Immunologie - Department of Immunology

Journal title :

PLOS ONE

Pages :

e0147871

Publisher :

Public Library of Science

Publication date :

2016-02

ISSN :

1932-6203

HAL domain(s) :

Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Maladies infectieuses

English abstract : [en]

Cerebral Malaria (CM) is associated with a pathogenic T cell response. Mice infected by P. berghei ANKA clone 1.49 (PbA) developing CM (CM +) present an altered PBL TCR repertoire, partly due to recurrently expanded T cell ...
Show more >Cerebral Malaria (CM) is associated with a pathogenic T cell response. Mice infected by P. berghei ANKA clone 1.49 (PbA) developing CM (CM +) present an altered PBL TCR repertoire, partly due to recurrently expanded T cell clones, as compared to non-infected and CM-infected mice. To analyse the relationship between repertoire alteration and CM, we performed a kinetic analysis of the TRBV repertoire during the course of the infection until CM-related death in PbA-infected mice. The repertoires of PBL, splenocytes and brain lympho-cytes were compared between infected and non-infected mice using a high-throughput CDR3 spectratyping method. We observed a modification of the whole TCR repertoire in the spleen and blood of infected mice, from the fifth and the sixth day post-infection, respectively, while only three TRBV were significantly perturbed in the brain of infected mice. Using multivariate analysis and statistical modelling, we identified a unique TCRβ signature discriminating CM + from CTR mice, enriched during the course of the infection in the spleen and the blood and predicting CM onset. These results highlight a dynamic modification and compartmentaliza-tion of the TCR diversity during the course of PbA infection, and provide a novel method to identify disease-associated TCRβ signature as diagnostic and prognostic biomarkers.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

ANR Project :

Sorbonne Universités à Paris pour l'Enseignement et la Recherche
Alliance française contre les maladies parasitaires
Phenomics en immunopathologie et inflammation: du cross-phenotypge aux biothérapies

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL