Titre :

Contribution of surface β-Glucan polysaccharide to physicochemical and immunomodulatory properties of Propionibacterium freudenreichii

Auteur(s) :

Deutsch, Stéphanie-Marie [Auteur correspondant]
Science et Technologie du Lait et de l'Oeuf [STLO]
Parayre-Breton, Sandrine [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Bouchoux, Antoine [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Guyomarc'H, Fanny [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Dewulf, Joëlle [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Dols, Marguerite [Auteur]
Œnologie
Baglinière, François [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Cousin, Fabien [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Falentin, Hélène [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Jan, Gwénaël [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Foligné, Benoit [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]

Titre de la revue :

Applied and Environmental Microbiology

Pagination :

1765-1775

Éditeur :

American Society for Microbiology

Date de publication :

2012

ISSN :

0099-2240

Mot(s)-clé(s) en anglais :

microscopie AFM
beta-glucan
polysaccharide

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Propionibacterium freudenreichii is a bacterial species found in Swiss-type cheeses and is also considered for its health properties. The main claimed effect is the bifidogenic property. Some strains were shown recently ...
Lire la suite >Propionibacterium freudenreichii is a bacterial species found in Swiss-type cheeses and is also considered for its health properties. The main claimed effect is the bifidogenic property. Some strains were shown recently to display other interesting probiotic potentialities such as anti-inflammatory properties. About 30% of strains were shown to produce a surface exopolysaccharide (EPS) composed of (1 -> 3,1 -> 2)-beta-D-glucan due to a single gene named gtfF. We hypothesized that functional properties of P. freudenreichii strains, including their anti-inflammatory properties, could be linked to the presence of beta-glucan. To evaluate this hypothesis, gtfF genes of three beta-glucan-producing strains were disrupted. These knockout (KO) mutants were complemented with a plasmid harboring gtfF (KO-C mutants). The absence of beta-glucan in KO mutants was verified by immunological detection and transmission electron microscopy. We observed by atomic force microscopy that the absence of beta-glucan in the KO mutant dramatically changed the cell's topography. The capacity to adhere to polystyrene surface was increased for the KO mutants compared to wild-type (WT) strains. Antiinflammatory properties of WT strains and mutants were analyzed by stimulation of human peripheral blood mononuclear cells (PBMCs). A significant increase of the anti-inflammatory interleukin-10 cytokine production by PBMCs was measured in the KO mutants compared to WT strains. For one strain, the role of beta-glucan in mice gut persistence was assessed, and no significant difference was observed between the WT strain and its KO mutant. Thus, beta-glucan appears to partly hide the anti-inflammatory properties of P. freudenreichii; which is an important result for the selection of probiotic strainsLire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Non spécifiée

Vulgarisation :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL