Title :

The Airway Microbiota in Cystic Fibrosis: A Complex Fungal and Bacterial Community-Implications for Therapeutic Management

Author(s) :

Delhaes, Laurence [Auteur]
Monchy, Sébastien [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Frealle, Emilie [Auteur]
Institut Pasteur de Dakar
Hubans, Christine [Auteur]
Salleron, Julia [Auteur]
Leroy, Sylvie [Auteur]
Prevotat, A. [Auteur]
Wallet, Frederick [Auteur]
Wallaert, Benoît [Auteur]
Dei-Cas, Eduardo [Auteur]
Sime-Ngando, Télesphore [Auteur]
Laboratoire Microorganismes : Génome et Environnement [LMGE]
Chabé, Magali [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Viscogliosi, Eric [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]

Journal title :

PLOS ONE

Pages :

e36313

Publisher :

Public Library of Science

Publication date :

2012-04

ISSN :

1932-6203

HAL domain(s) :

Planète et Univers [physics]/Sciences de la Terre/Océanographie

English abstract : [en]

Background Given the polymicrobial nature of pulmonary infections in patients with cystic fibrosis (CF), it is essential to enhance our knowledge on the composition of the microbial community to improve patient management. ...
Show more >Background Given the polymicrobial nature of pulmonary infections in patients with cystic fibrosis (CF), it is essential to enhance our knowledge on the composition of the microbial community to improve patient management. In this study, we developed a pyrosequencing approach to extensively explore the diversity and dynamics of fungal and prokaryotic populations in CF lower airways. Methodology and Principal Findings Fungi and bacteria diversity in eight sputum samples collected from four adult CF patients was investigated using conventional microbiological culturing and high-throughput pyrosequencing approach targeting the ITS2 locus and the 16S rDNA gene. The unveiled microbial community structure was compared to the clinical profile of the CF patients. Pyrosequencing confirmed recently reported bacterial diversity and observed complex fungal communities, in which more than 60% of the species or genera were not detected by cultures. Strikingly, the diversity and species richness of fungal and bacterial communities was significantly lower in patients with decreased lung function and poor clinical status. Values of Chao1 richness estimator were statistically correlated with values of the Shwachman-Kulczycki score, body mass index, forced vital capacity, and forced expiratory volume in 1 s (p = 0.046, 0.047, 0.004, and 0.001, respectively for fungal Chao1 indices, and p = 0.010, 0.047, 0.002, and 0.0003, respectively for bacterial Chao1 values). Phylogenetic analysis showed high molecular diversities at the sub-species level for the main fungal and bacterial taxa identified in the present study. Anaerobes were isolated with Pseudomonas aeruginosa, which was more likely to be observed in association with Candida albicans than with Aspergillus fumigatus.ConclusionsIn light of the recent concept of CF lung microbiota, we viewed the microbial community as a unique pathogenic entity. We thus interpreted our results to highlight the potential interactions between microorganisms and the role of fungi in the context of improving survival in CF.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL