Titre :

Feature of adhesins expressed in <em>Mycobacterium intracellulare</em>

Auteur(s) :

Lefrançois, Louise [Auteur]
Infectiologie et Santé Publique [UMR ISP]
Branger, Maxime [Auteur]
Infectiologie et Santé Publique [UMR ISP]
Cochard, Thierry [Auteur]
Infectiologie et Santé Publique [UMR ISP]
Peuchant, Olivia [Auteur]
Centre Hospitalier Universitaire de Bordeaux [CHU Bordeaux]
Raze, Dominique [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Locht, Camille [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Lanotte, Philippe [Auteur]
Infectiologie et Santé Publique [UMR ISP]
Biet, Franck [Auteur correspondant]
Infectiologie et Santé Publique [UMR ISP]

Titre de la manifestation scientifique :

European Symposium on Non-tuberculous Mycobacteria

Ville :

Borstel

Pays :

Allemagne

Date de début de la manifestation scientifique :

2015-06-24

Date de publication :

2015

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Microbiologie et Parasitologie

Résumé en anglais : [en]

The Mycobacterium avium MAC complex includes two closely related species Mycobacterium avium and Mycobacterium intracellulare (Mi). These non-tuberculous mycobacteria are opportunistic pathogens in humans and responsible ...
Lire la suite >The Mycobacterium avium MAC complex includes two closely related species Mycobacterium avium and Mycobacterium intracellulare (Mi). These non-tuberculous mycobacteria are opportunistic pathogens in humans and responsible of severe diseases in a wide variety of animals. These bacteria are also isolated from various environmental sources. In humans Mi is the second cause of illness after the strains of Mycobacterium tuberculosis complex. The genomic data available for the reference strain ATCC 13950 have enabled the development of genotyping but few studies concern identification of factors involved in the pathogenicity of this mycobacteria. The objective of this study was to identify, isolate and characterize the adhesins belonging to the family of HBHA and LBP from the reference strain and from a panel of clinical isolates from patients with different pathologies associated with Mi infection. Based on the genomic sequence of Mi we investigated whether the genes of HBHA and LBP were present and conserved between the different strains of the panel. The expression of adhesin HBHA and LBP in Mi was investigated by Western blot from lysates of human isolates Mi. An approach using affinity chromatography on heparin Sepharose was used to isolate and purify native adhesins from Mi. Our results allow for the first time the characterization of the expression of adhesins HBHA and LBP in Mi. This preliminary work opens numerous perspectives on the role of these molecules in human pathologies but also in diagnostic approaches of NTM infections in human.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL